Sources of anti-Haemophilus somnus antibody in bovine uterine secretions following intramuscular immunization and subsequent intrauterine inoculation of killed H. somnus were investigated. Holstein cattle (n = 21) were immunized with a 270-kDa outer membrane protein from H. somnus (omp-270) by intramuscular injection. At estrus, the cattle were given an intrauterine inoculum of a heat-killed su...

AIM:To develop a safe and effective DNA vaccine for inducing humoral and cellular immunological responses against hepatitis B virus surface antigen (HBsAg).METHODS:BALB/c mice were inoculated with NV-HB/s, a recombinant plasmid that had been inserted S gene of hepatitis B virus genome and could express HBsAg in eukaryotes. HBsAg expression was measured by ABC immunohis tochemical assay, generat...

Method: Fifteen nonpregnant gilts obtained from a PRRS-free herd were vaccinated twice with a modified-live PRRSV vaccine prior to artificial insemination. At 90 days of gestation, these VACC-CHAL gilts and 16 pregnant, nonvaccinated CHAL sows were randomly allotted to one of four experimental groups: a control group that received a sham inoculation, or to groups that received a “low” (102 CCID...

A viscous mucoid fluid occasionally accumulates after intratesticular inoculation of rabbits with Treponema pallidum. Experiments were performed to assess the effects of this testicular fluid on the development of syphilitic lesions. Intramuscular injections of this fluid altered host defences as indicated by shorter incubation periods, by reactivation of healing lesions, and by the presence of...

It has been reported that some strains of transplantable fowl leukosis are capable of inducing sarcomas at the site of inoculation when suitable material is injected intramuscularly or subcutaneously. Observations on this subject have been published bv Oberling and Gu&in (] 8,19, 20), Rothc Meyer and Engelbreth-Ilolm, for strain E.S., (22, 6), Stubbs and Furth, for strain 13, (2~). Histological...

It has been reported that some strains of transplantable fowl leukosis are capable of inducing sarcomas at the site of inoculation when suitable material is injected intramuscularly or subcutaneously. Observations on this subject have been published bv Oberling and Gu&in (] 8,19, 20), Rothc Meyer and Engelbreth-Ilolm, for strain E.S., (22, 6), Stubbs and Furth, for strain 13, (2~). Histological...

We have previously shown that tetracyclines interact with and reverse the protease resistance of pathological prion protein extracted from scrapie-infected animals and patients with all forms of Creutzfeldt-Jakob disease, lowering the prion titre and prolonging survival of cerebrally infected animals. To investigate the effectiveness of these drugs as anti-prion agents Syrian hamsters were inoc...