INTRODUCTIONThe MS2 system provides optimal sensitivity for single-molecule detection in cells. It requires two genetically encoded moieties: a reporter mRNA that contains MS2 binding site (MBS) stem loops and a fluorescent MS2 coat protein (MCP-xFP) that binds to the stem loops with high affinity, thus tagging the mRNA within the cell. This protocol describes transfection of COS-7 cells with r...

IntroductionThe rise of innovative technologies is having a transformative impact on contemporary society. Two types stand out. The first digital information and telecommunications, which has been developing ever more rapidly since the

IntroductionThe development of information and communication technologies is increasing rapidly. Technology essentially made to assist facilitate human activities. Still, sometimes it misused as a crime tool, especially during the COVID-19

During the 5-year period from 1980 to 1984, the incidence of subarachnoid hemorrhage due only to rupture of aneurysms was investigated in Izumo City, a small city with a population of 79,026. Additionally, to ascertain the relation of incidence to the size of the geographic area and/or population, a similar analysis was made on a larger area of Shimane Prefecture, including Izumo City, with a t...

To reduce overpopulation around Seoul, Korea, the government implemented a relocation policy of public officers by moving the government complex. This implies that there will be a negative impact on the suburban area that originally hosted the complex, but we do not know the magnitude of the impact. Therefore, this paper presents a micro-level estimation of the impact on the city commerce with ...

INTRODUCTIONThe labeling of entire plantlets (whole-mount labeling) is very easy, but it can cause substantial artifacts. Because whole plants are labeled, certain tissues are not readily accessible to the probes, whereas others can often stain nonspecifically. In the hands of the author, for instance, the elongation zone of the root is labeled even in negative controls, whereas mature parts of...

IntroductionThe aim of this study is to report the management of large periapical lesion using triple antibiotic paste as an alternative intra-canal medicament in nonsurgical endodontic treatment. Case reportan 11 year old male patient with large periapical lesion in mandibular anterior region, treated using conservative non-surgical endodontic treatment. Calcium hydroxide-iodoform paste (Vitap...