YggB and MscL are the major mechanosensitive channels in Escherichia coli, and each can rescue the double knockout mutant from osmotic downshock. However, the role of MscL in wild-type bacteria is in question, not only because cells without MscL survive severe osmotic downshocks, but because 1.8 times more suction is required to gate MscL than YggB under patch clamp. Here, we extend previous ev...

During healing of a skin wound, cells that are normally dormant must turn on hundreds of genes in order to proliferate, migrate, and repair the defect. The Polycomb family of proteins epigenetically silence target genes and so downregulation of their expression is a potential mechanism for unsilencing repair genes. Quantitative RT-PCR, western blotting, and immunostaining of invitro epithelial ...

During healing of a skin wound, cells that are normally dormant must turn on hundreds of genes in order to proliferate, migrate, and repair the defect. The Polycomb family of proteins epigenetically silence target genes and so downregulation of their expression is a potential mechanism for unsilencing repair genes. Quantitative RT-PCR, western blotting, and immunostaining of invitro epithelial ...

Emerging evidence suggests that neurogenesis may occur within the adult hypothalamus but the character and location of hypothalamic neural stem cells is unknown. We have identified a stem cell niche, confined to the central/posterior hypothalamus. Niche-derived cells require FGF signalling to maintain their proliferation exvivo, and proliferate, but do not differentiate, in response to exogenou...

Vediyuppu cheyaneer has been widely used in Siddha system of medicine for various diseases. The liquid form of Vediyuppu cheyaneer showed a significant inhibitory effect when screened at 500 mg/kg, for the invivo antiurolithiasis activity on ethylene glycol induced hyperoxaluria rats. The standard frusemide (20 mg/kg) and cystone (750 mg /kg) were used as comparison for diuretic and antiurolith...

Emerging evidence suggests that neurogenesis may occur within the adult hypothalamus but the character and location of hypothalamic neural stem cells is unknown. We have identified a stem cell niche, confined to the central/posterior hypothalamus. Niche-derived cells require FGF signalling to maintain their proliferation exvivo, and proliferate, but do not differentiate, in response to exogenou...

Emerging evidence suggests that neurogenesis may occur within the adult hypothalamus but the character and location of hypothalamic neural stem cells is unknown. We have identified a stem cell niche, confined to the central/posterior hypothalamus. Niche-derived cells require FGF signalling to maintain their proliferation exvivo, and proliferate, but do not differentiate, in response to exogenou...

canarium l. is used in folk medicine as anti-inflammatory, antibacterial, antifungal, antitumor, heptoprotective, antioxidant, and anti diabetic properties. the present study investigeated the invivo anti-inflammatory activity of  essential oil obtained by hydrodistillation of resin of canarium strictum roxb. in the current study, anti-inflammatory activity of essential oil of canarium strictum...

In this study we have performed genome-wide analysis to identify Nkx2-5 binding regions using mid-gestation mouse hearts to gain insight into the molecular mechanisms by which Nkx2-5 contributes to the correct development of the heart. Nkx2-5 and SRF invivo binding sites were mapped using ChIPchip assays and analysis of the ChIP-chip data has identified 600 and 858 SRF and Nkx2-5-binding sites,...