Real-time and embedded systems have traditionally been designed for closed environments where operating conditions, input workloads, and resource availability are known a priori and are subject to little or no change at runtime. There is an increasing demand, however, for autonomous capabilities in open distributed real-time and embedded (DRE) systems that execute in environments where input wo...

BACKGROUND Environmental contamination in hospitals with antibiotic-resistant organisms (AROs) is associated with patient contraction of AROs. This study examined the working relationship of Infection Prevention and Control (IPAC) and Environmental Services and the impact of that relationship on ARO rates. METHODS Lead infection control professionals completed an online survey that assessed t...

While enteroinvasive Escherichia coli (EIEC) and shigellae are genotypically nearly identical, a difference has been reported in the infective dose to humans: EIEC is 10,000-fold less infectious than shigellae. A possible basis for this difference lies in the inherent invasiveness of these bacteria toward epithelial cells. Thus, despite the high degree of homology between the invasion plasmids ...

Tn5-tagged invasion plasmid DNA (pWR110) from Shigella flexneri serotype 5 (strain M90T) was cloned into the expression vector lambda gt11. Recombinant phage (lambda gt11Sfl) expressing pWR110-encoded polypeptide antigens were identified by using rabbit antisera directed against S. flexneri M90T invasion plasmid antigens. Antigens encoded by lambda gt11Sfl recombinant phage were characterized b...

P. N. Appleton SIRTF Science Center, MS 220-6, California Institute of Technology, Pasadena CA 91125 V. Charmandaris Astronomy Department, Cornell University, 106 Space Sciences Bldg, Ithaca NY 14853 Yu Gao IPAC, MS 100-22, Infrared Processing & Analysis Center, Caltech, Pasadena CA 91125 & Department of Astronomy, University of Massachusetts, LGRT-B 619E, 710 North Pleasant Street,Amherst, MA ...

Diarrheal diseases still remain health problem worldwide and out of many bacteria responsible for, Shigella and pathogenic Escherichia cause the most diarrheas in the world. Shigellacause bacterial dysenteries and shigellosis through invasion where the most effective proteins for pathogenesis is Ipac. Critical virulence protein for ETEC infection is CFA/I with two subunits called cfab and cfae....

We discuss the networking dimension of the Integrated Platform for Autonomic Computing (IPAC). IPAC supports the development and running of fully distributed applications that rely on infrastructureless (ad-hoc) network with multi-hop transmission capabilities. Such environment is typically used for the realization of collaborative context awareness where nodes with sensors “generate” and repor...

Shigella invades epithelial cells by inducing cytoskeletal reorganization localized at the site of bacterial-host cell interaction. During entry, the Shigella type III secretion apparatus allows the insertion of a pore that contains the IpaB and IpaC proteins into cell membranes. Insertion of this complex is thought to allow translocation of the carboxy-terminus moiety of IpaC, but also of othe...

We have previously cloned two distinct regions of the Shigella sonnei form I plasmid pSS120, a 37-kilobase-pair DNA region and a virF region, which were found to be essential for cell invasion in Escherichia coli K-12 (J. Kato, K. Ito, A. Nakamura, and H. Watanabe, Infect. Immun. 57:1391-1398, 1989). The 37-kilobase-pair DNA region was randomly inserted by use of transposon Tn3-lac. At least ei...

A facile, sustainable method for the selective modification of aliphatic hydroxy (R–OH) groups in Kraft lignin was developed using an ionic liquid, 1-ethyl-3-methylimidazolium acetate (EmimOAc), as a solvent and catalyst. Selective R–OH achieved by one-pot, two-step homogeneous reaction: (i) acetylation aromatic OH (Ar–OH) with isopropenyl (IPAc) acyl donor (ii) subsequent deacetylation generat...