نتایج جستجو برای: isothermal titration calorimetry
تعداد نتایج: 35048 فیلتر نتایج به سال:
To apply enzymes in technical processes, a detailed understanding of the molecular mechanisms is required. Kinetic and thermodynamic parameters enzyme catalysis are crucial to plan, model, implement biocatalytic processes more efficiently. While kinetic parameters, Km kcat, often accessible by optical methods, determination requires sophisticated methods. Isothermal titration calorimetry (ITC) ...
Current Isothermal Titration Calorimetry (ITC) data in the literature have relatively high errors in the measured enthalpies of protein-ligand binding reactions. There is a need for universal validation standards for titration calorimeters. Several inorganic salt co-precipitation and buffer protonation reactions have been suggested as possible enthalpy standards. The performances of several com...
Interaction between aspartic acid and D-glucose, D-galactose, and D-fructose has been studied by isothermal titration calorimetry, calorimetry of dissolution, and densimetry. It has been found that D-glucose and D-fructose form thermodynamically stable associates with aspartic acid, in contrast to D-galactose. The selectivity in the interaction of aspartic acid with monosaccharides is affected ...
Detailed stopped-flow studies in combination with site-directed mutagenesis, isothermal titration calorimetry data and x-ray crystallographic knowledge have revealed that the biphasic pre-equilibrium fluorescence changes reported for a single Ig-binding domain of protein L from Peptostreptococcus magnus binding to kappa light chain are due to the binding of the kappa light chain at two separate...
The ability of the jack bean lectin concanavalin A (ConA) to bind seven membered ring (septanose) monosaccharides has been investigated by isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR spectroscopy.
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