BACKGROUND Antinuclear antibodies (ANA) giving a rim-like/membranous (RL/M) or a multiple nuclear dot (MND) pattern are highly specific for primary biliary cirrhosis (PBC). Aim and SUBJECTS To assess the prevalence of PBC specific ANAs, their Ig isotype, and their clinical significance in 90 PBC patients from Greece and Spain. Twenty eight patients with chronic hepatitis C, 23 patients with s...

Immune complexes containing human gamma (g)l or murine g2a antibodies generate secondary erector mechanisms via Fc receptor binding or complement activation, whereas those containing human g4 or murine gl antibodies generally do not. Therefore, isotype selection of therapeutic antibodies may have important clinical consequences. In a rabbit model of recombinant human tumor necrosis factor (rhuT...

Immunoglobulin (Ig) isotype (IgG, IgG1, IgG2, IgG3, IgG4, IgM, IgD and IgE) levels were investigated, both pre- and post-treatment with praziquantel (PZQ), in 43 adults and children chronically infected with Schistosoma mansoni, by means of a two-site, isotype-specific immunoenzymometric assay. The patients were classified as responders (R) or non-responders (NR) on the basis of their circumova...

Supplementary Figure 1: Isotype control of NKG2DL on medium treated PAM2.12 cells. Representative histogram profiles of medium treated PAM2.12 cells stained with isotype control or Mult-1, Rae-1 and H60 specific Ab. Samples were analyzed by FACS.

Although IgG2a is the most potent Ab isotype in the host response to viral and bacterial infections, the regulation of class switch recombination to IgG2a in vivo is not yet well understood. Recognition of pathogen-associated molecular patterns by dendritic cells expressing TLRs, like TLR7, recognizing ssRNA, or TLR9, recognizing DNA rich in nonmethylated CG motifs (CpG), favors induction of Th...

Immune complexes containing human gamma (g)1 or murine g2a antibodies generate secondary effector mechanisms via Fc receptor binding or complement activation, whereas those containing human g4 or murine g1 antibodies generally do not. Therefore, isotype selection of therapeutic antibodies may have important clinical consequences. In a rabbit model of human tumor necrosis factor (rhuTNF)-induced...

BACKGROUND Recently, autoantibodies against novel UH-RA peptides (UH-RA.1 and UH-RA.21) were identified as candidate biomarkers for patients with rheumatoid arthritis (RA) who are seronegative for the current diagnostic markers rheumatoid factor and anticitrullinated protein antibodies. Previously, screening for anti-UH-RA autoantibodies was based on measuring the immunoglobulin (Ig) G response...

Immune complexes containing human gamma (g)l or murine g2a antibodies generate secondary erector mechanisms via Fc receptor binding or complement activation, whereas those containing human g4 or murine gl antibodies generally do not. Therefore, isotype selection of therapeutic antibodies may have important clinical consequences. In a rabbit model of recombinant human tumor necrosis factor (rhuT...

The tumor necrosis factor family members BAFF and APRIL induce Ig isotype switching in human B cells. We analyzed the ability of BAFF and APRIL to induce isotype switching in murine B cells to IgG1, IgA, and IgE. APRIL and BAFF each engage two receptors, transmembrane activator and calcium-modulator and cytophilin ligand interactor (TACI) and B cell maturation antigen (BCMA), on B cells. In add...

TROP2 is highly expressed in breast cancer. antibody drug conjugates (ADCs) have shown antitumor activity cancer, however the determinants of response remain controversial. Datopotamab deruxtecan (Dato-DXd) an ADC consisting a humanized anti-TROP2 IgG1 monoclonal covalently linked to potent topoisomerase I inhibitor payload via stable, tumor-selective, tetrapeptide-based cleavable linker. We as...