molecular characterization of 19 jatropha accessions that included 15 accessions of j.curcas and 4 different species was carried out using 3 different markers systems. highest polymorphism (96.67%) was recorded by rapd followed by damd (91.02%) and issr (90%). polymorphism information content (pic) was higher for damd (0.873) and almost equal for rapd (0.863) and issr (0.862) markers, whereas r...

BACKGROUND Gymnema sylvestre, a vulnerable plant species, is mentioned in Indian Pharmacopeia as an antidiabetic drug. OBJECTIVE Study of genetic and chemical diversity and its implications in accessions of G. sylvestre. MATERIALS AND METHODS Fourteen accessions of G. sylvestre collected from Central India and assessment of their genetic and chemical diversity were carried out using ISSR (i...

This study was conducted to assess the efficacy of SCoT and DAMD molecular markers in genetic diversity  of three moss species and compare them with ISSR marker. Another objective was to evaluate the suitability of these DNA markers in species identification (delimitation) in three moss species, including Neckera complanata, Homalothecium sericeum and Neckera cris...

Planting resistant varieties is the most effective control measure against the angular leaf spot of dry beans, a fungal disease caused by Pseudocercospora griseola. However, dry bean varieties with durable resistance are not easily obtained. Knowledge about the genetic variability of the pathogen population is key for the success of dry bean breeding programs aimed at developing resistant ...

Native diversity is well represented in northern and eastern parts of India for mango. We evaluated three important polymerase chain reaction (PCR) based marker techniques viz., random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and directed amplified mini satellite DNA (DAMD) and examined their suitability for depicting genetic relationships and discrimination among c...

The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs is added to the PCR reaction and the PCR products are resolved on PAGE and auto...

the effectiveness of irap, remap, ssr, and issr markers were investigated to assess genetic diversity among and within eight medicago sativa l. populations. a total of 101, 119, 117 loci and 31 alleles were amplified using 10 irap, 14 remap, 16 issr and eight ssr primers, respectively. irap markers generated the maximum proportion of polymorphic loci per primer (pplp) while the maximum value of...

Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The I...

micropropagated plantlets derived from three different grape rootstock genotypes namely, dogridge (vitis champini), so4 (v. berlandieri×v. rupestris) and ari-h-144 (v. vinifera×v. labrusca) were subjected to randomly amplified polymorphic dna (rapd) and inter simple sequence repeats (issr) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...