Drug solubility plays an important role in the improvement of bioavailability. Biopharmaceutical Classification System (BCS) Class-II drugs have less and more permeability. Most available market belong to BCS class-II. The objective present study is improve drug, Cilnidipine by formulating them as solid dispersions make controlled release formulations. Solid were prepared solvent evaporation te...

•TRIP12 facilitates PROTAC-induced and CRL2VHL-mediated degradation of BRD4•TRIP12 specifically assembles K29-linked Ub chains•TRIP12 CRL2VHL cooperatively assemble K29/K48-branched chains on BRD4•Targeted uses unique mechanisms rather than a simple “hijack” Targeted protein is an emerging therapeutic paradigm. Small-molecule degraders such as proteolysis-targeting chimeras (PROTACs) induce the...

The polysaccharide capsule of Escherichia coli K29 fully surrounds the microorganism and thus occupies an extracellular space ca. 20 times larger in volume than that of the decapsulated cell. Since more than 95% of the capsule consists of water, dehydration for electron microscopy causes the material to collapse. We describe here a method for embedding the capsule in an uncollapsed form. Dehydr...

Polyubiquitination is a critical protein post-translational modification involved in a variety of processes in eukaryotic cells. The molecular basis for selective recognition of the polyubiquitin signals by cellular receptors is determined by the conformations polyubiquitin chains adopt; this has been demonstrated for K48- and K63-linked chains. Recent studies of the so-called non-canonical cha...