Keratin 7 (K7) is a Type II member of the keratin superfamily and despite its widespread expression in different types of simple and transitional epithelia, its functional role in vivo remains elusive, in part due to the lack of any appropriate mouse models or any human diseases that are associated with KRT7 gene mutations. Using conventional gene targeting in mouse embryonic stem cells, we rep...

To study the role of the amino-terminal domain of the desmin subunit in intermediate filament (IF) formation, several deletions in the sequence encoding this domain were made. The deleted hamster desmin genes were fused to the RSV promoter. Expression of such constructs in vimentin-free MCF-7 cells as well as in vimentin-containing HeLa cells, resulted in the synthesis of mutant proteins of the...

Background; Feather waste is generated in large amounts as a by-product of commercial poultry processing. The main component of feather is keratin. The main purpose of this study was to identify Bacillus spp. (the keratinolytic bacteria) that are able to degrade the feather for producing keratin. Methods; Bacillus spp. Were isolated from the waste of poultries located in Miyaneh city. The bact...

Studies of transverse sections of osmiumand leadstained a-keratin in the electron microscope led to the suggestion (1) that the microfibril (about 75A in diameter) contained protofibrils about 20A in diameter . Johnson and Sikorski (2) criticized this conclusion on the grounds that the highresolution image of a microfibril in transverse section should not be regarded as a simple projection of t...

Keratin expression in human cervical squamous cell carcinoma (SCC) lines differed significantly from both normal and human papillomavirus (HPV) immortalized exocervical cells. Keratin 14 (K14) expression, determined by protein synthesis and mRNA levels, was dramatically down-regulated in the cervical SCC lines while keratin 5 (K5) expression was not. K14 expression was similarly down-regulated ...

[PDF] [Full Text] [Abstract] , May 24, 2001; 344 (21): 1580-1587. N. Engl. J. Med. N.-O. Ku, R. Gish, T. L. Wright and M. B. Omary Keratin 8 Mutations in Patients with Cryptogenic Liver Disease [PDF] [Full Text] [Abstract] , August 20, 2001; 154 (4): 763-774. J. Cell Biol. S. Gilbert, A. Loranger, N. Daigle and N. Marceau protection occurs through a receptor-targeting modulation Simple epit...

Cytokeratins in taste buds were immunocytochemically evaluated with monoclonal antibodies. In each of six different epithelial sites in the rat oral cavity, intragemmal cells of taste buds were immunoreactive for keratin polypeptides 8, 18, and 19, as well as for keratin 7, which has not been previously reported in taste buds. Keratin-18-like immunoreactivity was present in fewer than half of t...

Oral squamous cell carcinoma is one of the most common oral malignant tumors comprising of 90-95 % of cases (Pindborg et al., 1990; Harrison et al., 1999). It is commonest malignancy in Indian sub continent (Pindborg et al., 1990; Parija, 1991). It is histologically graded as well, moderate and poorly differentiated tumor based on several factor (Neville et al., 2002’ Neville et al., 2002). One...

Recently we generated keratin 10 knockout mice which provided a valuable model for the dominantly inherited skin disorder epidermolytic hyperkeratosis. Here we investigated the molecular basis for their phenotype. Hetero- and homozygotes expressed a truncated keratin 10 peptide which has been identified directly by microsequencing. Epitope mapping of monoclonal antibodies to keratin 10T enabled...

objective(s): response surface methodology (rsm) by central composite design (ccd) was applied to statistically optimize the preparation of rutin-quercetin (ru-qr) dual drug loaded human hair keratin nanoparticles as well as evaluate the characteristics. materials and methods: the effects of three independent parameters, namely, temperature (x1:10-40 c), surfactant (x2: sds (1), sls (2), tween-...