Keratinolytic abilities of Bacillus polymyxa B20 and B. cereus B5esz were evaluated in liquid cultures in mineral media containing chicken feathers. Both tested strains were capable of effective liquefying and biodegradation of feather keratin, up to 56.5 – 72.1% in ten-day cultures, releasing considerable amounts of hydrolysis products. Tested bacteria were mesophilic species, producing highes...

The keratinolytic potential of the kearatinolytic fungi was determined by the release of protein by utilizing feather keratin. The total protein yield was found to be 430 ug mL -1 from Chrysosporium indicum, 410 ug mL -1 protein from Microsporum canis, 390 ug mL -1 protein from Crysosporium keratinophillum, 370 ug mL -1 protein from Microsporum gypseum, 360 ug mL -1 protein from Trichophyton ru...

The data on the occurrence of keratinolytic fungi in sewage, municipal solid waste and waste-contaminated habitats are reviewed. In these habitats, keratinolytic fungi occur with extreme abundance. The factors influencing qualitative and quantitative compositions of the fungi in the habitats are discussed. It is concluded that keratinolytic fungi can be the bioindicators of environmental pollut...

A novel alkaliphilic Nocardiopsis sp., strain TOA-1, was isolated from a tile-joint of a bathroom. Strain TOA-1 produced a variety of alkaline hydrolytic enzymes. An alkaline protease, designated NAPase, was purified and characterized. NAPase had a very high keratinolytic activity and high stability under acidic conditions.

Feathers are byproduct waste of poultry processing plant and produced in large amount. A small percentage of feather waste is steamed, chemically treated, ground, to form feather meal a dietary protein supplement for animals. Alternatively, keratin can be biodegraded by some Keratinolytic bacteria and in this study Keratinase producing bacteria and their Keratinolytic enzyme production was inve...

Due to the content of pathogenic organisms, including fungi, sewage sludge land application poses risks to both human health and the environment. One of the ways for reducing pathogens in sludge is liming. This study aims to determine the effect of sludge liming on the composition of keratinolytic and keratinophilic fungi in model experiments. The fungi were examined using the hair baiting meth...

Chryseobacterium sp. strain P1-3, harboring keratin degrading activity, has recently been isolated from poultry waste. Here, we report the 4.6-Mbp draft genome sequence of the keratinolytic bacterium with a G+C content of 37.0% and 4,087 protein-coding genes.

Based on previous screening for keratinolytic nonpathogenic fungi, Paecilomyces marquandii and Doratomyces microsporus were selected for production of potent keratinases. The enzymes were purified and their main biochemical characteristics were determined (molecular masses, optimal temperature and pH for keratinolytic activity, N-terminal amino acid sequences). Studies of substrate specificity ...

The complete genome sequence of Chryseobacterium camelliae Dolsongi-HT1 is reported here. C. camelliae Dolsongi-HT1, having keratinolytic activity, was isolated from green tea leaves in the Dolsongi tea garden in Jeju, South Korea. The strain Dolsongi-HT1 has 28 candidate protease genes, which may be utilized in further studies and industrial applications of keratinase.

Strain T1E, isolated and identified as Brevibacillus thermoruber, and evolutionally distant from the known keratinolytic isolates, proved to have feather-degrading ability. During the 7-day fermentation period, T1E consumed 10 g/l native goose feathers as the sole source of carbon and energy at 50 degrees C under aerobic conditions. The isolate secreted a thermostable, keratinolytic protease, w...