Nearly every pathologist that practices in a community hospital setting struggles with the accurate and reproducible identification of HPV effect and dysplasia in the female genital tract, and recognizes the fact that in certain situations these conditions can be very difficult to distinguish from reactive changes. Last month in the ProPath "Focus on Immunohistochemistry", we reviewed the poten...

An underlying theme common to prominent theoretical accounts of cognition in schizophrenia is that information processing is disproportionately influenced by recently/currently encountered information relative to the influence of previously learned information. In this study, the authors tested this account by using the hindsight bias or knew-it-all-along (KIA) paradigm, which demonstrates that...

An improved immunoenzymatic double labelling method was developed, which simultaneously shows the Ki-1 membrane antigen and the nuclear proliferation associated antigen, defined by monoclonal antibody Ki-67. This new approach permits in situ discrimination of cells that are proliferating or not with a particular membrane antigen. Most Ki-1 positive cells in normal lymphoid tissue also express t...

BACKGROUND Phyllodes tumors (PT) of the breast are uncommon, and it is often difficult to predict their clinical behavior from histologic features in individual cases. In addition to routine morphology, the studies of p53 protein and Ki-67 antigen expression in PT may be useful to differentiate benign from malignant tumors. METHODS Immunohistochemical analyses using monoclonal antibody to lab...

The study aimed at determining levels of metallothionein (MT) and Ki-67 antigen expression in adenocarcinomas of large intestine and examining relation of the expression levels with various clinical and pathological variables. The studies were performed on 81 cases of large intestine adenocarcinoma. Using immunocytochemistry, expressions of MT (positive reaction in 73 cases) and of Ki-67 (posit...

Antigen Ki-67 is a non-histone nuclear protein, closely connected with cell proliferation. Determining Ki-67 allows for a quick and reliable evaluation of the growth fraction of the studied cell population. It serves as a marker of proliferative activity in neoplasms and other diseases with excessive cell proliferation such as psoriasis. Evaluation of Ki-67 expression in epidermal cells was per...

Thyroid carcinomas are the most frequently occurring tumours in the endocrine system. Metallothioneins (MT) and Ki-67 proteins are present in intensely proliferating cells, and their expression has been observed in numerous tumours, including thyroid tumours. The purpose of this study was to analyse the relationship between intensity of MT expression and Ki-67 antigen on one hand and histologic...

The applicability of MIB-1, a monoclonal antibody directed against the Ki-67 antigen, was studied in the PC-82 and LNCaP prostatic tumor models at various levels of proliferative activity. Statistically significant correlations were found in LNCaP cultures between Ki-67 and MIB-1 scores (r = 0.84, P < 0.001), and in PC-82 tumors between MIB-1 scores and paraffin tissue Ki-67 (pKi-67) (r = 0.90,...

In progression of tumours, resulting from, i.e., release of cells from the parental tumour and development of metastases, expression of cell adhesion molecules (CAM) plays a significant role. CAM, including E-cadherin and the linked to it β-catenin, determine the extent of adhesion between normal and neoplastically altered cells. Moreover, the unbound form of β-catenin in a cell nucleus may aff...

In progression of tumours, resulting from, i.e., release of cells from the parental tumour and development of metastases, expression of cell adhesion molecules (CAM) plays a significant role. CAM, including E-cadherin and the linked to it beta-catenin, determine the extent of adhesion between normal and neoplastically altered cells. Moreover, the unbound form of beta-catenin in a cell nucleus m...