The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucida...

Uncontrolled proliferation and apoptosis evasion are two hallmarks of acute myeloid leukemia (AML), but the molecular mechanisms remain poorly understood. In this study, it is demonstrated that double over-expresser oncoprotein c-Myc anti-apoptotic protein Bcl-2 a critical “genetic overdrive” in AML associated with poor genetic alterations. Double-knockdown c-Myc/ synergistically kills cells vi...

Abstract Problem Does aquaporin 3 (AQP3) affect the migration and invasion of human extravillous trophoblast (HTR8/Svneo) cells? Method study A lentivirus infection system was used to construct stable cell lines with either AQP3 knockdown or overexpression. RT-PCR western blotting were verify efficiencies overexpression in HTR8/Svneo cells at mRNA protein levels, respectively. Cell Counting Kit...

The treatment of prostate cancer patients usually starts with androgen ablation and followed by chemotherapy; however, in some cases the tumor develops resistant phenotype. Combination therapy is currently regarded as a cornerstone in cancer therapy to overcome the drug resistance. Herein, we investigated the combinatory effect of Docetaxel and Trastuzumab with a novel nanomedicine, BCc1. Also,...

New promising insect control efforts are now being evaluated such as biological alternatives or even transgenic insects and Wolbachia based strategies. Although it is increasingly clear that successful approaches must involve integrated actions, chemical insecticides unfortunately still play a central role in pest and vector control (Raghavendra et al., 2011). Development of new safe and effect...

iv CHAPTER 1. General Introduction 1 CHAPTER 2. Literature Review 5 CHAPTER 3. Rac2 expression in the zebrafish (Danio rerio) 25 CHAPTER 4. Rac2 function in the adult zebrafish neutrophil 45 CHAPTER 5. The effect of Rac2 inhibition and gene knockdown during infectious disease on the mortality of zebrafish (Danio rerio) embryos infected via immersion 61 CHAPTER 6. General Conclusions 75