We developed a bacteria-based AND logic gate using a Pseudomonas aeruginosa lasI/rhlI double mutant with two quorum-sensing signaling molecules as the input signals. We showed a distinct electrical output signal, despite the complexity and continuous regulation of metabolic reactions of living cells.

Cell-to-cell signaling controls many virulence genes in Pseudomonas aeruginosa. We tested the virulence of las and rhl quorum-sensing mutants in neonatal mice. A lasI rhlI double mutant was nearly avirulent, and the respective single mutant strains were reduced in virulence compared with the wild-type strain. Quorum sensing plays a role in P. aeruginosa pneumonia in neonatal mice.

Background Influence of marital status on cognitive risk remains understudied in India with 17.7% the global population. Methods To examine influence (categorized as married, never widowed and other which included partnered, separated or divorced) to what extent is a protective factor for decline. For this observational study design, we merged nationally representative harmonized Longitudinal A...

Cholecystokinin (CCK)-A and CCK.B/gastrln receptors were evalu ated with in vitro receptor autoradlography in 406 human tumors of various origins using a sulfated lasI@labelod CCK decapeptide analogue lasI.([email protected], Me@1).CCK 26-33 and *asI.Inbelod Leu15-gastrin as radioligands. CCK-B/gastrln receptors were found frequently in medul lat@r thyroid carcinomas (92%), In small cell lung cancers (...

Two Gram-negative, plant growth-promoting rhizobacteria (PGPRs), denominated as M12 and M14, were classified by 16S rDNA sequencing as Burkholderia graminis species. Both strains were shown to produce a variety of N-acyl-homoserine lactone (AHL) quorum sensing (QS) signalling molecules. The involvement of these molecules in plant growth promotion and the induction of protection against salt str...

In response to diverse environmental stimuli at different infection sites, Pseudomonas aeruginosa, a serious nosocomial pathogen, coordinates the production of different virulence factors through a complicated network of the hierarchical quorum-sensing (QS) systems including the las, rhl, and the 2-alkyl-4-quinolone-related QS systems. We recently showed that at early stages of growth serum alt...