B-cell acute lymphoblastic leukaemia (B-ALL) reprograms the surrounding bone marrow (BM) stroma to create a leukaemia-supportive niche. To elucidate contribution of immune cells leukaemic microenvironment, we investigated involvement monocyte/macrophage compartments, as well several recruitment pathways in B-ALL development. Immunohistochemistry analyses showed that CD68-expressing macrophages ...

-A study has been made of the thymidine labelling index (TLI) of marrow blast cells in 201 adults with untreated acute leukaemia. There was no significant difference between the TLI in 172 patients with acute myeloblastic leukaemia and 29 patients with acute lymphoblastic leukaemia. The TLI did not correlate with the age, sex, peripheral-blood or marrow blast-cell count, or the platelet count a...

Acute myeloid leukaemia (AML) is a heterogeneous neoplastic disorder in which a subset of cells function as leukaemia-initiating cells (LICs). In this study, we prospectively evaluated the leukaemia-initiating capacity of AML cells fractionated according to the expression of a nucleolar GTP binding protein, nucleostemin (NS). To monitor NS expression in living AML cells, we generated a mouse AM...

OBJECTIVE To determine the frequency of rearrangement of the myeloid-lymphoid leukaemia gene in acute leukaemia in Hong Kong Chinese children. DESIGN Immunophenotyping, cytogenetic, and molecular analysis. SETTING Regional hospital, Hong Kong. PARTICIPANTS Bone marrow or peripheral blood samples were taken from 27 children aged 16 years or younger with acute leukaemia, from September 1995...

In the western part of the Netherlands during 1973-80 leukaemia was diagnosed in 293 patients aged under 15 years. An overall incidence rate of 2.91 per 100000 person years was calculated. No seasonal influence on months of birth or months of diagnosis of these patients could be traced by the method of Edwards. Time space clustering was looked for by both methods of Mantel and Knox. No signific...

AMONG sixteen cases of acute leukaemia with orbital leukaemic infiltration in children, there were six myeloblastic, six monoblastic, two lymphoblastic (Mortada 1962, 1963, 1964a), one undifferentiated stem-cell, and one aleukaemic leukaemia. The following case of lymphoblastic aleukaemic leukaemia is described because bilateral proptosis in this condition has been only rarely reported, and bec...

Bone marrow erythroid progenitor cells were examined from 50 cases of acute leukaemia and from 20 normal subjects using an in vitro semisolid culture method. Numbers of both primitive erythroid progenitor cells (BFU-e) and later-stage erythroid progenitor cells (CFU-e) were remarkably depressed in patient with acute leukaemia in active phase. However, both BFU-e and CFU-e recovered to within no...

Occurrences of second malignancies in hairy cell leukaemia are well recognised. Most of these malignancies are either solid tumours or lymphoproliferative disorders. The association of myeloproliferative disorders with hairy cell leukaemia (HCL) is very rare. This report describes a case of a patient with HCL who after remaining in remission developed Philadelphia chromosome positive chronic my...

We investigated the influence of recombinant human tumour necrosis factor alpha (TNF-alpha) and its derivatives termed muteins III, V, VI-in which the first 3 to 7 amino acids of native TNF-alpha have been replaced-on the survival time of mice inoculated with leukaemia L1210 or leukaemia P338. TNF-alpha prolonged the survival of mice with leukaemia L1210 but did not have any therapeutic activit...

Patients with 47, XXY karyotype (Klinefelter syndrome) appear to have increased risk of developing cancer, especially male breast cancer, germ cell tumours and non Hodgkin lymphomas, but rarely acute myeloid leukaemia. We report a patient with acute basophilic leukaemia with 47, XXY karyotype in both the tumour and constitutional cells. Acute basophilic leukaemia is very rare disease comprising...