Candida parapsilosis is a human pathogenic fungus with increasing importance, particularly in nosocomial infections. For detailed molecular genetic explorations of prototrophic clinical isolates of C. parapsilosis, we developed an efficient transformation system based on a dominant selectable marker. The gene encoding resistance to mycophenolic acid (MPA) was used for selection in yeast transfo...

Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S. cerevisiae to other important yeast species is discussed. The protocols for transformation by s...

Motion information can form the basis of predictions about time-toimpact and the trajectories of objects moving through a scene. Firstly, a model that incorporates accumulative computation and lateral interaction is presented. By means of the lateral interaction in accumulative computation (LIAC) of each element with its neighbours, the model is able to segment moving objects present in an inde...

Transformation efficiencies for Pichia pastoris are usually several orders of magnitude below those for other yeast. We report here that pretreatment of P. pastoris with 0.1 M lithium acetate (LiAc) and 10 mM dithiothreitol (DTT) before electroporation increased transformation efficiency approximately 150-fold. DTT alone enhanced the transformation efficiency up to 20-fold, but LiAc alone had l...

In this chapter we have provided instructions for transforming yeast by a number of variations of the LiAc/SS-DNA/PEG method for a number of different applications. The rapid transformation protocol is used when small numbers of transformants are required. The high efficiency transformation protocol is used to generate large numbers of transformants or to deliver DNA constructs or oligonucleoti...

In I Z patients with idiopathic liaciiiocliroiiiatosis aiid varying dcgrces of iroii ovcrload the scru~ii fcrritin coiicctitratioii was rclatcd to the aiiiouiit of iron mobilizcd by vciicsectioit. Tlicrc was 110 corrclation Lctwceii iron loadaiidcliclatablc iron. In 35 patients with normal iron storcs following vciic~cctioii therapy, both transfcrrin iron and cliclatablc iron was pathologically...

background: one of the main problems of dedicated iort accelerators is to determine dosimetric characteristics of the electron beams. monte carlo simulation of iort accelerator head and produced beam will be useful to improve the accuracy of beam dosimetry. materials and methods: liac accelerator head was modeled using the beamnrcmonte carlo simulation system. phase-space files were generated a...