Abstract Background Infestation by tea green leafhoppers ( Empoasca (Matsumurasca) onukii ) can cause a series of biochemical changes in leaves. As typical cell-rupture feeder, E. secretes proteases while using its stylet to probe the tender shoots plants Camellia sinensis ). This study identified and analyzed expressed specifically salivary gland (SG) gut through enzymatic activity assays comp...

There is emerging evidence that the proteolytic machinery of plants plays important roles in defense against pathogens. The oomycete pathogen Phytophthora infestans, the agent of the devastating late blight disease of tomato (Lycopersicon esculentum) and potato (Solanum tuberosum), has evolved an arsenal of protease inhibitors to overcome the action of host proteases. Previously, we described a...

A reverse transcription-polymerase chain reaction (RT-PCR) strategy was used to clone diverse trypsin-like protease gene transcripts from midguts of the brown planthopper Nilaparvata lugens Stål (Homoptera: Delphacidae). Six individual trypsin-like protease transcripts were identified. On the basis of one nucleotide sequence of the six clones, a full-length cDNA sequence (1902 bp) was obtained ...

The protease enzyme produced by Lactic Acid Bacteria (LAB) with characteristics similar to rennin is called Rennin Like Protease (RLP). purpose of the study was obtain information on biochemical RLP Enterococcus faecium 1.15 as a basis for utilization enzyme. first stage observation growth curves and potential isolates produce RLP, characterization. (RLP) which has been purified using Sephadex ...

Cysteine proteases are an important class of enzymes implicated in both developmental and defense-related programmed cell death and other biological processes in plants. Because there are dozens of cysteine proteases that are posttranslationally regulated by processing, environmental conditions, and inhibitors, new methodologies are required to study these pivotal enzymes individually. Here, we...

the aim of this study was to determine the inhibitory effects of feed ingredients on protease activities of argyrosomus regius larvae using in vitro techniques. a. regius larvae fed on a commercial feeding procedure were sampled thirteen times, during the sampling period (from 3 to 32 days after hatching (dah)). the differences observed in protease activities of meagre larvae during the samplin...

M1 myeloid leukemic cells overexpressing wild-type p53 undergo apoptosis. This apoptosis can be suppressed by some cytokines, protease inhibitors, and antioxidants. We now show that induction of apoptosis by overexpressing wild-type p53 is associated with activation of interleukin-1beta-converting enzyme (ICE)-like proteases, resulting in cleavage of poly(ADP- ribose) polymerase and the proenzy...