Correction After publication of this work [1], it came to our attention that the laboratory we use changed the method of analysis for insulin and C-peptide during our data collection. Before March 2007 the method used for insulin was competitive radioimmunoassay (Linco Research Inc, St Charles, MO, USA) and for C-peptide competitive luminoimmunoassay (Immulite 2000, Diagnostic Products Corporat...

OBJECTIVE Adiponectin, an adipocyte-secreted hormone, circulates in the serum in several multimeric forms. Compared with total adiponectin, high-molecular weight (HMW) adiponectin has been suggested to be a better predictor of metabolic parameters and insulin sensitivity in humans. Our objective was to compare total adiponectin with HMW adiponectin as predictors of metabolic variables and insul...

After equilibrating cells with [32P]phosphate there were 3.7 f 0.2 mol of [32P]phosphate/mol of Band 2 subunit; this is the total measured as nonradioactive phosphate in fresh cells (Harris, H. W., Wolfe, L. C., and Lux, S . E. (1978) Fed Roc. 37, 1507). This level can be reduced in intact cells by metabolic depletion, or on purified spectrin by tryptic removal of the terminal phosphorylated pe...

After equilibrating cells with [32P]phosphate there were 3.7 f 0.2 mol of [32P]phosphate/mol of Band 2 subunit; this is the total measured as nonradioactive phosphate in fresh cells (Harris, H. W., Wolfe, L. C., and Lux, S . E. (1978) Fed Roc. 37, 1507). This level can be reduced in intact cells by metabolic depletion, or on purified spectrin by tryptic removal of the terminal phosphorylated pe...

Saprolegnia ferax contains an integrin homologue, identified by crossreactivity with antiserum to the consensus sequence of human/chick/Xenopus cytoplasmic domain beta 1-integrin, which is highly conserved. In non-reduced samples, this integrin was larger than the reported size range for beta 1-integrins, at 178 kDa. In reduced samples, there was a reducing agent-concentration-dependent convers...

After equilibrating cells with [32P]phosphate there were 3.7 f 0.2 mol of [32P]phosphate/mol of Band 2 subunit; this is the total measured as nonradioactive phosphate in fresh cells (Harris, H. W., Wolfe, L. C., and Lux, S . E. (1978) Fed Roc. 37, 1507). This level can be reduced in intact cells by metabolic depletion, or on purified spectrin by tryptic removal of the terminal phosphorylated pe...

The life threatening anemia in beta-thalassemia major (Cooley's anemia) is characterized by profound intramedullary lysis, the cause of which is incompletely understood. Using marrow obtained from beta thalassemia major patients undergoing allogeneic bone marrow transplantation in Pesaro Italy, it became possible to directly study the mechanism of the intramedullary hemolysis. Based on our prev...

The Linco-Bennett auditory response cradle is a microprocessor controlled device for screening the hearing of neonates. A total of 396 neonates admitted to a special care unit were tested on the cradle and later followed up in a comprehensive test programme between the ages of 3 months and 8 months. Altogether 374 (94%) were available for follow up. The use of the cradle resulted in the detecti...

OBJECTIVE Adiponectin may play a role in the development of type 2 diabetes and cardiovascular disease (CVD). However, little is known about the relationship between adiponectin and impaired glucose tolerance (IGT). We investigated the association between adiponectin and IGT and between adiponectin and cardiovascular risk factors among subjects with IGT. RESEARCH DESIGN AND METHODS Subjects w...

OBJECTIVE Some neuropeptides and monoaminergic neurotransmitters may affect hypothalamic feeding centres, sympathetic activity and thermogenesis. Sibutramine (BTS54524; N-[1-[1(4-chloro phenyl) cyclobutyl]-3methyl N,N-dimethylamine hydrochloride monohydrate) is a new 5-HT serotonin and noradrenaline reuptake inhibitor (SNRI), antiobesity drug. The aim of this study was to evaluate the effects o...