To attain muscle continuity with a minimal scar in the treatment of the microform cleft lip, the current author previously introduced a new method using interdigitation of the orbicularis oris muscle to create a philtrum through an intraoral incision to avoid an external scar.1 A total of 12 patients with microform cleft lip were treated between August of 2001 and October of 2002. The follow-up...

Synthesis of multiple extracellular lipases in Candida rugosa has been demonstrated. However, it is difficult to characterize the expression spectrum of lip genes, since the sequences of the lip multigene family are very closely related. A competitive reverse transcription-PCR assay was developed to quantify the expression of lip genes. In agreement with the protein profile, the abundance of li...

Lipases are currently the subject of intensive studies due to their large range of industrial applications. The Lip2p lipase from the yeast Yarrowia lipolytica (YlLIP2) was recently shown to be a good candidate for different biotechnological applications. Using a combination of comparative genomics approaches based on sequence similarity, synteny conservation, and phylogeny, we constructed the ...

Background Lignin is a complex aromatic polymer whose microbial degradation is essential for carbon recycling [1]. The fungus Ceriporiopsis subvermispora has attracted attention for its valuable biotechnological applications, especially for pretreatment of lignocellulosic material studies in order to expose the cellulose for hydrolysis and industrial fermentation. Thus, ligninolytic enzymes of ...

BACKGROUND In recent years, the non-conventional model yeast species Yarrowia lipolytica has received much attention because it is a useful cell factory for producing recombinant proteins. In this species, expression vectors involving LIP2 and POX2 promoters have been developed and used successfully for protein production at yields similar to or even higher than those of other cell factories, s...

Yarrowia lipolytica (YL) is a "non-conventional" yeast that is capable of producing important metabolites. One of the most important products that is secreted by this microorganism is lipase, a ubiquitous enzyme that has considerable industrial potential and can be used as a biocatalyst in the pharmaceutical, food, and environmental industries. In this work, Yarrowia lipolytica lipase (YLL) was...