Diurnal changes in glycogen stores of adipose tissues and in vitro lipolytic activity of isolated epididymal fat cells, and their lipolytic responsiveness to epinephrine and theophylline were examined in rats adapted to a 2-h daily meal feeding (20.00-22.00 h; darkness between 20.00-08.00 h) for 3 weeks and in control rats fed ad libitum. The fat cells from both groups of animals showed the pea...

The effect of bioaugmentation with an anaerobic lipolytic bacterial strain on the anaerobic digestion of restaurant lipid-rich waste was studied in batch experiments with a model waste containing 10% lipids (triolein) under two sets of experimental conditions: (A) methanogenic conditions, and (B) initially acidogenic conditions in the presence of only the lipolytic strain biomass (4 days), foll...

The in vitro lipolytic response to norepinephrine by rat adipocytes from epididymal, subcutaneous, perirenal, mesenteric, and omental tissue sites was studied in control and hypotensive animals. Lipolysis per millimole of triglyceride was found to be three to four times higher in mesenteric and omental fat cells than in adipocytes of the other sites sampled. The high lipolytic activity of mesen...

Eurycomanone (1) and 13β,21-epoxyeurycomanone (2) were isolated from Eurycoma longifolia for studies of lipolytic activity. Compound 1 enhanced lipolysis in adipocytes with an EC50 of 14.6μM, while its epoxy derivate, compound 2, had a stronger activity with an EC50 of 8.6μM. Based on molecular mechanistic study using several specific inhibitors to lipolytic signaling pathways, it was found tha...

Studying lipase in germinating sunflower seedlings, we looked for an activator of the lipolytic activity. In the presence of 1.25 mM ATP, the enzyme activity increased 2-fold. Lipid-body lipase solubilization was realized using two detergents: Tween 80 and CHAPS. Lipolytic activity was increased 10-fold in the presence of 2% (w/v) CHAPS, showing the probable 'complexity' of the enzyme. Looking ...

An extract of ovine and bovine pineal glands (labeled Precipitate II) has previously been shown to possess lipolytic activity on rabbit adipose tissue. This extract also causes darkening of the frog’s skin in vivo and in vitro. Both lipolytic and melanotropic activities are abolished by trypsin and chymotrypsin. Fractionation of ovine pineal Precipitate II by the sequence, chromatography on die...

Ayeast isolate, selected for its lipolytic activity from a meat product, was characterized as Pichia anomala. Lipolytic activity, determined on p-NPA as esterase, was maximum at 28 ◦C, pH 6.5, and induced by the short chain triglyceride tributyrin. Fermentations in 2 L and 10 L stirred tank bioreactors, with 20 and 60 g/L glucose respectively, showed that in the second case lipolytic activity i...

The intravascular injection of heparin results in the rapid appearance in the blood of a lipolytic enzyme capable of clearing lipemic sera and hydrolyzing artificial triglyceride emulsions (1, 2). This enzyme has been termed clearing factor (3), lipoprotein lipase (4), clearing factor lipase (2), or postheparin plasma lipolytic activity (PHLA) (5). A lipolytic enzyme with characteristics nearly...

This study was aimed at evaluating the capability of Yarrowia lipolytica W29 for the synthesis of lipolytic enzymes in a medium containing plant oils from non-conventional sources with some components displaying bioactivity. Oils from almond, hazelnut, and coriander seeds were obtained by using n-hexane (Soxhlet method) and a chloroform/methanol mixture of solvents (Folch method), and their eff...

The present study was undertaken to determine the relationship between the antilipolytic and lipolytic effects of insulin on hormone-stimulated lipolysis and the mechanisms of these reactions. The dose-response curve of norepinephrine-stimulated lipolysis in rat adipocytes was not sigmoidal but biphasic in nature. Intracellular free fatty acid levels were linearly related to lipolytic rate and ...