The protein McaP was previously shown to be an adhesin expressed by the Moraxella catarrhalis strain O35E, which also displays esterase and phospholipase B activities (J. M. Timpe et al., Infect. Immun. 71:4341-4350, 2003). In the present study, sequence analysis suggests that McaP is a conventional autotransporter protein that contains a 12-stranded beta-barrel transporter module (amino acids ...

Lipolytic bacteria were isolated from oil contaminated soils and grown on tributyrin media containing 1% (w/v) olive oil. The isolate showing maximum activity was identified by following Berger’s manual. Different media parameters were optimized for maximal enzyme production. Peak lipase activity was observed for palm oil as carbon source, peptone as nitrogen source, at pH 7.0 and temperature a...

Chryseobacterium mulctrae KACC 21234T is a novel species isolated from raw bovine milk. Psychrotrophic bacteria are considered contaminants and hypothesized to originate the environment. In this investigation, C. genome was determined be 4,868,651 bp long assembled into four contigs with G+C ratio of 33.8%. silico genomic analyses revealed presence genes encoding proteases (endopeptidase Clp, o...

1. A number of rumen bacteria isolated because of their ability to deacylate phosphatidyl choline, were found, in addition, to hydrogenate polyunsaturated fatty acids. 2. The most active lipolytic organisms had an unusual pattern of hydrogenation of dietary fatty acids in that alpha-linolenic acid was hydrogenated only as far as trans-11, cis-15-octadecadienoic acid.

The contamination of bulk samples of cow’s raw milk (n = 491) by psychrotrophic lipolytic bacteria (PLiBC), total count of psychrotrophic bacteria (PBC) and mesophilic bacteria (TBC) was monitored for two years on eight dairy farms and the correlations among these groups of bacteria were analysed. An increase in TBC, PBC and PLiBC and in the values of free fatty acids (FFA) was tested experimen...