various basidiomycetes and deuteromycetes, grown in liquid and solid culture media, were compared for their laccase-producing ability and for the inducing effect of soil and agricultural residues on laccase production. laccase activity in extracts of all solid media was higher than that of the liquid media. in liquid cultures, fungal laccase activity in ex-tracts of pea (pisum sativum) straw-tr...

The ionic composition of the fluid lining the airways (airway surface liquid, ASL) in healthy subjects and patients with cystic fibrosis (CF) has been a matter of controversy. It has been attempted to resolve conflicting theories by using cell cultures, but published results show a wide variety of values for the ionic concentrations in the apical fluid in these cultures. To investigate CFTR-med...

Intracellular pH (pH(i)) was measured during the circadian cycle of Neurospora. Internal pH of Neurospora cultures in liquid medium was assayed by the 5,5-dimethyl-2,4-oxazolidinedione method and gave values for pH(i) which were similar to those previously obtained by other workers using pH-microelectrodes with agar-grown cultures. Cytoplasmic pH changed in liquid medium cultures, but these cha...

Stationary cultures of Pleurotus ostreatus, MTCC-1804 supplemented with bagasse powder or its ethanolic extract, showed a concentration-dependent enhancement in the laccase activity both in liquid and semi-solid (glass bead) cultures. Bagasse extract also imparted greater stability to the enzyme. Mn-peroxidase (MnP) activity was detected only in cultures supplemented with bagasse or its extract...

A long-term liquid culture system of hemopoietic tissue derived from adult hamster spleen has been described. These primary liquid cultures can maintain stem cell proliferation and differentiation for more than three months without secondary repopulation. A characteristic of the liquid cultures is the formation of clusters of hemopoietic cells around adherent stromal cells. Some islands were co...