We compared the development of B-cell progenitors with that of myeloid progenitors in fetal liver cells at various gestational ages. Day 12 to 14 fetal liver cells did not form pre-B-cell colonies. Pre-B-cell colonies were developed from day 15 fetal liver cells. The incidence of colonies increased with increases in gestational age and reached a maximum on days 18 to 19. In contrast, the incide...

Although the expression of Pitx2, a bicoid family homeodomain transcription factor, is highly regulated during hematopoiesis, its function during this process was not documented; we thus studied hematopoiesis in Pitx2-null mice. We found that Pitx2(-/-) embryos display hypoplastic livers with reduced numbers of hematopoietic cells, but these cells had normal hematopoietic potential, as evidence...

background: bone marrow stromal cells (bmsc) have been successfully employed for movement deficit recovery in spinal cord injury (sci) rat models. one of the unsettled problems in cell transplantation is the relative high proportion of cell death, specifically after neural differentiation. according to our previous studies, p75 receptor, known as the death receptor, is only expressed in bmsc in...

conclusions our findings showed that the treatment of flaps with bm-msc and aam transplantations significantly promoted flap survival compared to a control group. the viability of the flap was improved by combining bm-mscs with aam matrix scaffolds. materials and methods bm-mscs and aams were transplanted into subcutaneous tissue in the flap area. on the 7th postoperative day, the surviving fla...

Precursor cells of the B lineage can be enriched from mouse fetal liver by FACS with the aid of the pre-B cell-specific mAb G-5-2. The cells are concomitantly enriched for cells expressing the pre-B cell-specific gene lambda 5, and for cells developing to LPS-reactive mature B cells. The enriched purified precursors are not influenced by rIL-2 through -7, alone or in combination, to develop to ...

In earlier studies on chimeric animals, we found that fetal thymocytes produced peripheral T lymphocyte populations depleted of CD4+ cells. This occurred whether the fetal thymocytes matured in the presence of adult or fetal thymic stromal cells. In contrast, fetal liver cells that differentiated in the adult thymus generated normal proportions of peripheral CD4+ cells. Because fetal liver cell...

objective(s): umbilical cord blood-derived mesenchymal stromal cells (ucb-mscs) are ideally suited for use in various cell-based therapies. we investigated a novel induction protocol (nip) to improve the neuronal differentiation of human ucb-mscs under appropriate conditions. materials and methods: this experimental study was performed in iranian blood transfusion organization (ibto), tehran, i...

objective(s): intracerebral injection of bone marrow stromal cells (bmscs) is being investigated as a therapeutic tool to prevent alzheimer's disease (ad). our aim was to investigate the effects of bmscs by intrathecal injection in ad rat model. materials and methods: bmscs were obtained from the bone marrow of wistar rat and transplanted into ad rat model via intrathecal injection. the ra...

the aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. blastocyst stage embryos from pregnant nmri mice were obtained and cultured for 1-2 days in dmem medium. the inner cell masses formed...

background: endometriosis is a complex estrogen-dependent disease that is defined as the presence of endometrial gland and stroma outside the uterine cavity. although the exact mechanism for the development of endometriosis remains unclear, there is a large body of research data and circumstantial evidence that suggests a crucial role of estrogen in the establishment and maintenance of this dis...