Abstract Therapeutic monoclonal antibodies (mAbs) are typically manufactured using mammalian cell cultures in fed‐batch bioreactors, with increasing emphasis on meeting productivity and product quality attribute targets that depend strongly such process variables as nutrient feed rates bioreactor operating conditions. In this article, we identify, categorize, address the challenges of achieving...

Mixing has been one of the important parameters in characterizing and designing external air-lift bioreactors for the biological processes that require intensive mixing for mass transfer. In this study, pH tracer method was employed to characterize the mixing quality of a three-phase laboratory-scale external-loop air-lift bioreactor (EALR) used for cultivation of a pelletized filamentous funga...

A dissolved methane sensor based on silicone tube was designed, constructed and optimized.The silicone tube diameter, silicone tube length and helium flow rate (as the carrier gas(were considered as process parameters to be optimized.A continuous stream of helium (50 mL/min) was directed through the tubing, sweeping out the...

Steady state and dynamic fermentations of baker's yeast in molasses based media were analyzed in a laboratory scale bioreactor. Sugar and biomass balances together with the Monod biokinetics were used to develop the process model. Parameters of the model were obtained using collected experimental data. Model predicted open loop responses to step changes in feed concentration as well as dilu...

steady state and dynamic fermentations of baker's yeast in molasses based media were analyzed in a laboratory scale bioreactor. sugar and biomass balances together with the monod biokinetics were used to develop the process model. parameters of the model were obtained using collected experimental data. model predicted open loop responses to step changes in feed concentration as well as dilution...

Limited available water resources have rendered wastewater reuse an important issue to specialists in most developed countries, today. The current study works on membrane filtration for treatment of industrial wastewater. By comparing the two methods of membrane bioreactor (MBR) and hybrid membrane electro bioreactor (MEBR) processes, it finds that earlier fouling in the membrane occurs in the ...

We describe the characterization of a chip-based platform (3(D)-KITChip) for the three-dimensional cultivation of cells under perfusion conditions via magnetic resonance imaging (MRI). Besides the chip, the microfluidic system is comprised of a bioreactor housing, a medium supply, a pump for generating active flow conditions as well as a gas mixing station. The closed circulation loop is ideall...

In the present work, removal of 4-chlorophenol (4-CP) by the mixed microbial consortium was evaluated in an airlift inner loop bioreactor. During the study, the effect of various reactor parameters such as hydraulic retention time (HRT), biogenetic substrate concentration, loading rate, and initial substrate concentration on the removal efficiency of 4-CP was investigated. Bioreactor showed a m...

We have developed an integrated array of microbioreactors, with 100 microL working volume, comprising a peristaltic oxygenating mixer and microfluidic injectors. These integrated devices were fabricated in a single chip and can provide a high oxygen transfer rate (k(L)a approximately 0.1 s(-1)) without introducing bubbles, and closed loop control over dissolved oxygen and pH (+/-0.1). The syste...

The development of energy-efficient solutions for large-scale fermenters demands a deep and comprehensive understanding hydrodynamic heat mass transfer processes. Despite wide variety research dedicated to measurements intensity in bubble flows, this subject faces new challenges due the topical innovative bioreactor designs. In order understand fluid dynamics gas–liquid medium, researchers need...