A mixture of the aliphatic alcohol, triacontanol, and other chemically associated naturally occurring alcohols was applied to the denuded dorsal cutaneous surface of guinea pigs to evaluate anti-inflammatory activity. In the setting of a chemical irritation with 2% croton oil and in an allergic dermatitis created with dinitrochlorobenzene sensitization and challenge, the triacontanol-containing...

Full blood count and erythrocyte sedimentation rate, antistreptolysin titre, albumin/ globulin ratio, blood sugar estimation, gonococcal complement-fixation test, Wassermann and Treponema pallidum immobilization tests, Waaler-Rose latex-fixation test, Toxoplasma complement-fixation test, Tine test, histoplasmosis complement-fixation test. Urine and stool examination for parasites and bacteria. ...

Twenty patients with sickle cell disease (14 black females and 6 black males, mean age 31.5 +/- 9.3) were studied by quantitating peripheral T and B lymphocyte percentages and measuring lymphocyte blast transformation (LBT) in response to phytohemagglutinin-P, concanavalin-A and pokeweed mitogen. Compared to normal black controls (19 black females and 1 black male, mean age 32.0 +/- 9.2 years) ...

After rejection of tissue allografts, animals acquire immunological memory to the transplantation antigens of the graft (1). The memory thus acquired manifests itself in several distinct reactions: (a) Accelerated rejection of a second graft antigenically similar to the first transplant (second set effect) (1). (b) Enhanced rejection of tissue grafts by normal animals receiving lymphocytes from...

In a lymphocyte blast transformation assay, the response of feline lymphocytes to concanavalin A was suppressed 20 to 65 percent in the presence of inactivated feline leukemia virus. The decrease was not due to viral cytotoxicity, as determined by trypan blue viability counts, nor was the virus binding the concanavalin A and interfering with its mitogenic stimulation. The virus may be biochemic...

Lewis strain rat lymphocytes were exposed in vitro to a variety of specific and nonspecific blastogenic stimuli. The surfaces of the transformed lymphocytes were examined by indirect immunofluorescence for the presence of T cell antigens and immunoglobulin molecules. More than 90% of lymphocytes that underwent blast transformation after exposure to foreign histocompatibility antigens (mixed lym...

E ARLY WORKERS considered the small lymphocyte in peripheral blood as an end-stage cell with limited biologic activity.1 In 1959, Bond et al.2 employing radioactive nucleoside precursors for DNA, substantiated the concept that the normal peripheral blood lymphocyte was a “resting” cell. Subsequently, Nowell3 in 1960 observed that a plant lectin from Phaseolous vulgarus, phytogemagglutimin ( PHA...

Short-term culture of acute myelogenous leukemia patient's remission lymphocytes with inactivated autologous leukemic blast cells plus allogeneic lymphocytes, generated effector T lymphocytes which were cytotoxic for the specific autologous blast cell in 11 of 14 patients studied. Experiments using Daudi and Molt 4 lymphoblastoid cell lines as third-party helper cell suggest that an HLA D locu...

Lymphocytes from tmsensitized animals or humans damage allogeneic cells in tissue culture when aggregated to the latter by means of phytohemagghtinin (PHA) (1-3). Under the experimental conditions used, injury of the target cells was not seen or was slight in the absence of PHA. When the PHA-treated lymphocytes were separated from the target cells by a Millipore falter, cell damage was prevente...

Dendritic cells (DC) confer concanavalin A responsiveness to accessory cell-depleted canine lymphocytes. DC-lymphocyte cluster formation is followed by blast transformation and proliferation of lymphocytes. We investigated whether fibronectin, which is known to be involved in cell:cell interactions, might also be involved in the interaction between canine DC and lymphocytes. The addition of rab...