We describe a new method of complete analysis for lecithin, lysolecithin, and sphingomyelin in amniotic fluid. The analysis is based on alkaline hydrolysis of the lecithins and the enzymic hydrolysis of sphingomyelin. The choline formed in each instance is enzymically phosphorylated with [gamma-32P]ATP to yield [gamma-32P]phosphorylcholine, which is isolated by anion-exchange chromatography and...

Evidence is presented that lysolecithin is involved in the fusing and hemolytic activities of Sendai virus. Treatment of the virus with phospholipase B (lysolecithinase) specifically inactivates the hemolytic and fusing abilities, without affecting the infectivity and the capability of the virus to hemagglutinate and adsorb to cells. The possible identity of lysolecithin with the "cell fusion f...

Duodenogastric reflux of bile acids and lysolecithin in the course of a standard test meal was measured in normal people and in patients with duodenal ulcer before operation and more than one year after highly selective vagotomy, Polya partial gastrectomy, truncal vagotomy and pyloroplasty, and truncal vagotomy and gastrojejunostomy. Before operation, duodenal ulcer patients had significantly h...

The electrogenic proton-translocating ATPase in the plasma membrane of Neurospora has been solubilized with lysolecithin and purified using a combination of gel filtration and density gradient centrifugation. Isolated plasma membrane vesicles are solubilized with lysolecithin in the presence of MgATP, vanadate, and chymostatin. The MgATP and vanadate are required to maintain the ATPase in an ac...

The precise mechanism underlying the common 'physiological' jaundice of the newborn remains uncertain (Goldbloom and Gottlieb, 1929; Billing and Lathe, 1956; Lathe and Walker, 1957; Nelson, 1964). This study was based upon a previous suggestion that increased haemolytic activity operates in some cases, and that an increased urinary potassium concentration is one expression of this, continuing a...

The incorporation of [2-3H]glycerol and lysolecithin labeled with [2-3H]glycerol into lecithin and its subspecies was investigated in epithelial and muscular layers of rat small intestine. The labeled compounds were administered in two wways: the intraduodenal injection and the injection into femoral vein. The incorporation of glycerol into lecithin was distinctly higher in epithelial layer tha...

The site of action of lecithinase A, which removes a single fatty acid from a glycerophospholipide molecule, has never been proved. The only report known to the author on this subject was that of Levene and Mehltretter (l), who were unsuccessful in their attempts to make a derivative of lysolecithin which had been prepared by the action of snake venom on egg lecithin. The availability of pure, ...

Lysolecithin and sphingomyelin may be readily separated from other phospholipids on thin-layer plates impregnated with silver nitrate using the solvent system chloroform-methanol-concentrated ammonium hydroxide 65:35:8. Quantitation is carried out by determining the phosphorus content of the developed bands. The minimum amount of phospholipid that can be quantitatively determined is that which ...