conclusions: pcr method is a valuable, cost-effective and alternative tool for quick diagnosis of active tuberculosis in different clinical specimens. results: the concordance rate between the three sets of primers was calculated and is6110/buffer pcr method showed good agreement with the lj culture method (κ = 0.627, p < 0.0001). the sensitivity of is6110/buffer pcr was 58.33%, with specificit...

in most countries, tuberculosis caused by mycobacterium bovis is mainly a disease of cattle but can infect buffalos too. the disease can be controlled successfully by mean of a test-and-slaughter program. in iran test-and-slaughter program has started since 1971 and prevalence of bovine tuberculosis reduces from 5% to less than 0.12% in recent years. in western azarbaijan, north west of iran, t...

anti-adhesion therapy represents a potentially promising avenue for the treatment and prevention of tuberculosis in a post-antibiotic era. adhesins are surface-exposed microbial structures or molecules that enable pathogenic organisms to adhere to host surfaces, a fundamental step towards host infection. although several mycobacterium tuberculosis adhesins have been identified, it is predicted ...

persons infected with the human immunodeficiency virus (hiv) are particularly susceptible to tuberculosis, either by latent infection reactivation or by a primary infection with rapid progression to active disease. this study was done to determine the frequency of tuberculosis infection among iranian patients with hiv/aids. a total of 262 hiv/aids patients attending all three hiv/aids health ca...

background: tuberculosis (tb) is a major cause of death worldwide. finding an effective vaccine against tb is the best way to control it. several vaccines against this disease have been developed but none are completely protective. the aim of this study was to design and construct a cloning vector containing the mycobacterium tuberculosis (m. tuberculosis) heat shock protein x (hspx). methods: ...

Objective(s): Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of Mycobacterium tuberculosis (M. tuberculosis) from the site of primary infection. Superior expression systems are required to obtain abundant M. tuberculosis proteins for the purpose of diagnosing M. tuberculosis infection or for the immuniza...

Cutaneous tuberculosis (cTB) represents 1-2% of extrapulmonary [1] and is caused by the Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, africanum, microti, canetti, caprae, pinnipedii).

background: transmission of mycobacterium tuberculosis ( m. tuberculosis ) can occur in different ways. furthermore, drug resistant in m. tuberculosis family is a major problem that creates obstacles in treatment and control of tuberculosis (tb) in the world. one of the most prevalent families of m. tuberculosis is haarlem, and it is associated with drug resistant. our objectives of this study ...

tuberculosis is an infectious disease caused by mycobacterium tuberculosis and is the primary cause of mortality in the world. m. tuberculosis strains are important intracellular pathogens causing infection in oxygen-rich macrophages in the lungs. the emergence of aids, declined socioeconomic standards and a lessened emphasis on tb control programs have contributed to the disease resurgence. re...

a nested polymerase chain reaction (pcr) assay with two pairs of primers was applied for the detection of m. tuberculosis complex dna in specimens of 51 patients. of these, 31 clinically diagnosed patients (group i) recruited from july 1996 to june 1997 were given antituherculous chemotherapy from one to six months with standard daily regimen (isaniazid, 5 mg/kg; rifampin, 10 mg/kg; pyrazinamit...