For the manufacturing of complex biopharmaceuticals using bioreactors with cultivated mammalian cells, high product concentration is an important objective. The phenotype cells in a reactor plays role. Are clonal cell populations showing cell-specific growth rates more favorable than lines higher productivities or vice versa? Five Chinese hamster ovary were analyzed based on data 3-month-stabil...

The paper by Johnson et al. "Germ line stem cells and follicular renewal in the postnatal mammalian ovary" published in Nature on the 11th of March [1] suggests that a population of germ line stem cells exist within the adult mouse ovary and that oocyte numbers are regulated by a balance of activation of these cells and cell death. This paper should be viewed in its historical context as it rei...

Pre-natal and early post-natal ovarian development has become a field of increasing importance over recent years. The full effects of perturbations of ovarian development on adult fertility, through environmental changes or genetic anomalies, are only now being truly appreciated. Mitigation of these perturbations requires an understanding of the processes involved in the development of the ovar...

The effect of the polyene antibiotic nystatin, used in combination with several other antibiotics, on the membranes of Candida albicans SC#8169 (yeast) cells and of Chinese hamster ovary and 3T3 (mammalian) cells was investigated. It was concluded from determinations of the viability of the yeast cells and from measurements of deoxyribonucleic acid synthesis by the mammalian cells that nystatin...

Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...

Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...

At or early after birth, mammalian ovaries are filled with primordial follicles each composed by an oocyte blocked at the end of prophase I surrounded by a single layer of granulosa cells. The doctrine that female mammals are born with a finite number of oocytes fated to be exhausted with the age has been challenged by recent results claiming that new oocytes can be continuously formed in the p...

introduction:artifical sourace of radiation have increased worldwide average annual exposure of mankind and could especially affect rapidly dividing cells in gonads. since, radiation sensitivities of different components of primordial follicles, and the time required for their deplation have not been morphologically eatablished we decided to estimate the time required for depletion of primordia...

introduction: heavy ions are nucleus of elements of iron, argon, carbon and neon that all carry positive electrical charges. for these particles to be useful in radiotherapy they need to accelerated to high energy by more than thousand mega volts. also the cosmic environment is considered to be a complicated mixture of highly energetic photons and heavy ions such as iron. therefore, the health ...