Clearances of mannan antigen from Candida albicans and glucuronoxylomannan antigen from Cryptococcus neoformans were examined in nonimmune rabbits by using self-prepared latex agglutination tests. Injected intravenously, 20 mg of Candida mannan antigen was cleared from the serum with a half-life of approximately 2 h. In contrast, 20 micrograms of Cryptococcus glucuronoxylomannan antigen had a h...

A soil microorganism, designated Arfhrobacfer GJM-1, has been isolated which is capable of growing on Saccharomyces cerevisiae mannan as the carbon source. When grown on mannan, the microorganism secretes an a-mannosidase into the cultural medium. The enzyme is not found when mannose, glucose, or glycerol is substituted for mannan, but is present when oligosaccharides obtained by acetolysis of ...

A soil microorganism, designated Arfhrobacfer GJM-1, has been isolated which is capable of growing on Saccharomyces cerevisiae mannan as the carbon source. When grown on mannan, the microorganism secretes an a-mannosidase into the cultural medium. The enzyme is not found when mannose, glucose, or glycerol is substituted for mannan, but is present when oligosaccharides obtained by acetolysis of ...

INTRODUCTION Prompt diagnosis of candidaemia and invasive candidosis is crucial to the early initiation of antifungal therapy. The poor sensitivity of blood cultures (BCs) has led to the development of fungal glycan tests as a diagnostic adjunct. We analysed the performance of tests for the detection of circulating β-D-1,3-glucan (BDG) and mannan in the intensive care unit (ICU) setting. METH...

We have previously demonstrated the ability of human neutrophil myeloperoxidase to bind to mannan isolated from Candida albicans. Mannan may therefore be a primary component of the yeast cell wall which provides for binding of myeloperoxidase, a requirement potentially important for the candidacidal activity of the enzyme. In this report, we describe experiments to consider the relationship of ...

A soil microorganism, designated Arfhrobacfer GJM-1, has been isolated which is capable of growing on Saccharomyces cerevisiae mannan as the carbon source. When grown on mannan, the microorganism secretes an a-mannosidase into the cultural medium. The enzyme is not found when mannose, glucose, or glycerol is substituted for mannan, but is present when oligosaccharides obtained by acetolysis of ...

BACKGROUND H1N1 influenza viruses mutate rapidly, rendering vaccines developed in any given year relatively ineffective in subsequent years. Thus it is necessary to generate new vaccines every year, but this is time-consuming and resource-intensive. Should a highly virulent influenza strain capable of human-to-human transmission emerge, these factors will severely limit the number of people tha...

The yeast Saccharomyces cerevisiae X2180, the strain most commonly used for genetic studies on yeasts, was treated with the mutagen ethyl methanesulfonate, and mutants defective in the synthesis of cell wall mannan were isolated. The initial selection of mutants was made on the basis of their failure to agglutinate with antiserum that was specific for the mannotetraose side chain of the mannan,...

Precipitins to mannan and cytoplasmic antigens of three Candida species were determined in an unselected series of 289 non-pregnant women. Precipitins were present in 20% of sera of women with vaginal candidiasis, in 23% of women harbouring yeasts in the vagina without clinical signs of infection, and in 21% of women harbouring yeasts in sites other than the vagina. Of the 47 patients who react...

We have obtained evidence for two structurally and antigenically different Saccharomyces cerevisiae cell wall mannans. One, which occurs widely and is found in S. cerevisiae strain 238C, is already known to be a neutral mannan which yields mannose, mannobiose, mannotriose, and mannotetraose on acetolysis of the (1 --> 6)-linked backbone. The other, which was found in S. cerevisiae brewer's stra...