One well documented family of enzymes responsible for the proteolytic processes that occur in the extracellular matrix is the soluble and membrane-associated matrix metalloproteinases. Here we present the first theoretical model of the biochemical network describing the proteolysis of collagen I by matrix metalloproteinases 2 (MMP2) and membrane type 1 matrix metalloproteinases (MT1-MMP) in the...

objective(s) the aim of the present study was to compare the therapeutic indices of several agents used in treatment of inflammatory conditions which included: vitamin e, hydro-alcoholic extracts of glycyrrhiza glabra, matricaria aurea, dexamethasone, piroxicam and diclofenac using wehi-164 fibrosarcoma cells. materials and methods cytotoxicity evaluation was based on vital dye exclusion assay....

The ability of normal rabbit dermal fibroblasts to degrade films of type IV collagen and gelatin when stimulated by phorbol ester was shown to be dependent on the induction, secretion and activation of 95 kDa gelatinase B and the secretion and activation of 72 kDa gelatinase A and stromelysin. Degradation was inhibited by exogenous human recombinant tissue inhibitor of metalloproteinases-1, spe...

SW1353 chondrosarcoma cells cultured in the presence of interleukin-1, concanavalin A or PMA secreted procollagenase 3 (matrix metalloproteinase-13). The enzyme was detected in the culture medium by Western blotting using a specific polyclonal antibody raised against recombinant human procollagenase 3. Oncostatin M enhanced the interleukin-1-induced production of procollagenase 3, whereas inter...

In the course of lung cancer, normal cells are transformed into cancerous ones, and changes occur in microenvironment, including extracellular matrix (ECM), which is not only a scaffold for cells, but also reservoir cytokines, chemokines growth factors. Metalloproteinases (MMPs) among elements that enable ECM remodeling. The publication focuses on problem gene expression MMP2, MMP9 tissue inhib...

OBJECTIVE To evaluate the effects of aging on left ventricular (LV) geometry, collagen levels, matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) abundance, and myocardial fibroblast function. METHODS Young (3-month-old; n=28), middle-aged (MA; 15-month-old; n=17), and old (23-month-old; n=16) CB6F1 mice of both sexes were used in this study. Echocardiographic par...

although pyrimethamine (tindurin™) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. we design this study to evaluate its anti-tumoral effect on a model of cell line. methods: the cytotoxic influence of pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. the po...

Objective: The aim of this study is to compare the results histopathological examination uterus healthy cows and that have recovered from metritis. 
 Materials Methods: materials obtained slaughterhouse were divided into two groups: (no metritis; Group N; n=10) had metritis about 45 days ago (metritis; M; n=10). Sections taken both groups stained with hematoxylin eosin (H&E) for pathol...

Matrix metalloproteinases (MMPs) function in the remodeling of the extracellular matrix that is integral for many normal and pathological processes. The tissue inhibitor of metalloproteinases family, including tissue inhibitor of metalloproteinases-2 (TIMP-2), regulates the activity of these multifunctional metalloproteinases. TIMP family members are proteinase inhibitors that contain six conse...