The cryopreservation of immature GV oocytes by vitrification is offering attractive perspectives, since they represent an important pool of germ cells for women undergoing IVM cycles, or at risk of infertility for OHSS or PCOs, particularly those poor responders to gonadotropin stimulation, or in case of cancer. Therefore, it becomes necessary to optimize both cryopreservation technique as well...

The Pacific white shrimp Litopenaeus vannamei is an economically important penaeid species worldwide. Under farming conditions, the full ovarian maturation of L. generally depends on combination artificial ablation unilateral eyestalk and feeding high-quality natural diets, suggesting that nutrient accumulation to maturation. In this study, we aimed investigate gene expression related nutrition...

BACKGROUND Oocyte maturation and competence to development depends on its close relationship with cumulus cells (CCs). However, the maturation conditions of human cumulus-oocyte complexes (COCs) might affect gene expression in both oocyte and CCs. We thus compared the transcriptome profiles of CCs isolated from in vivo and in vitro matured COCs at different nuclear maturation stages. METHODS ...

Background We have previously reported the effect of Anthocyanin and cAMP modulators on maturation and apoptosis of sheep oocytes. In this study, we investigated the reduction capacity and apoptotic gene expression results at MII sheep oocytes. MaterialsAndMethods Good ovine Cumulus-oocyte complexes (COCs) were transferred to Pre-IVM medium, then to two type IVM mediums: 1) 0.1μg/ml Delphinidin...

The incidence of cardiovascular disease has been continuously increasing. Because cardiomyocytes (CM) are non-renewable cells, it is difficult to find appropriate CM sources repair injured hearts. Research human induced pluripotent stem cell (hiPSC) differentiation and maturation into invaluable for the treatment heart diseases. use hiPSCs as regenerative therapy allows many diseases that canno...

The maturation of induced pluripotent stem cells (iPS) is one of the limiting steps of somatic cell reprogramming, but the underlying mechanism is largely unknown. Here, we reported that knockdown of histone deacetylase 2 (HDAC2) specifically promoted the maturation of iPS cells. Further studies showed that HDAC2 knockdown significantly increased histone acetylation, facilitated TET1 binding an...

Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...

Background: Recent studies demonstrated the multifactorial and chronic nature of male infertility, including mutations of some known spermatogenesis-related genes. The H2B family, member W (H2B.W) gene is one of the testis specific histone variant genes that encodes a sperm telomere-binding protein, required for reorganization and integration of sperm chromosomes. The objective of the present s...

A cDNA-AFLP approach on Linum usitatissimum (flax) was used to identify genes specifically expressed during the seed maturation process. Among the 20,000 cDNA-AFLP tags produced, 486 were selected for their seed-specific expression during maturation. When compared with the publicly available databases, half of them presented some significant similarity with known plant sequences. The results ob...

Abstract Background Mutations in the LMNA gene encoding Lamin A/C, a major component of nuclear lamina, cause laminopathies including dilated cardiomyopathy (DCM). DCM patients with mutations have particularly severe clinical courses such as heart transplantation and death due to failure. However, underlying mechanisms LMNA-induced remains elusive. Methods results We identified Q353R mutation f...