Large-scale screening of hundreds or even thousands of crystallization conditions while with low sample consumption is in urgent need, in current structural biology research. Here we describe a fully-automated droplet robot for nanoliter-scale crystallization screening that combines the advantages of both automated robotics technique for protein crystallization screening and the droplet-based m...

A new approach to enhancing proton conductivity of ceramics is demonstrated by aligning proton conductive pathways and eliminating resistive grain boundaries. Hydroxyapatite (HAP) membranes are synthesized by multistage crystallization onto palladium. The synthesis involves three steps: electrochemical deposition of HAP seeds, secondary hydrothermal crystallization onto the seed layer to promot...

Protein crystallization techniques are very important in drug development. This paper discusses a membrane-based micro-volume dialysis method suitable for rapid and high-throughput protein crystallization. A droplet of solution was deposited on the membrane surface device. Crystallizing agents could be added to either directly or through membrane. The process easily monitored real time under mi...

In this work, low-cost and high permeation NaA zeolite membrane was successfully prepared on kaolinite support by in-situ crystallization using secondary growth method. The effect of synthesis conditions including temperature time crystallization, water ratio studied aiming to improve the quality performance membrane. thoroughly characterized many techniques such as X-ray diffraction, scanning ...

effluent from ro (reverse osmosis) systems during recovery gradually becomes concentrated and supersaturated with soluble salts such as calcium carbonate (caco3), calcium sulfate (caso4), barium sulfate (baso4) and silica (sio2). thus we were looking for procedures to prevent precipitation of soluble salts on the membrane surface and decrease membrane permeability. therefore, recovery could be ...

The second virial coefficient, or B value, is a measurement of how well a protein interacts with itself in solution. These interactions can lead to protein crystallization or precipitation, depending on their strength, with a narrow range of B values (the 'crystallization slot') being known to promote crystallization. A convenient method of determining the B value is by self-interaction chromat...

Chiral resolution will increase its importance in the pharmaceutical, pesticide, and food industries since future chiral products be structurally more complex while their quality need to meet stringent demands accomplish with health, crop, environmental purposes. Currently, obtaining enantiopure crystals from racemic compound-forming systems (90–95% of compounds) remains a challenge. The combin...

This paper utilizes cyclodextrin-based host-guest chemistry in a microfluidic device to modulate the crystallization of membrane proteins and the process of concentration of membrane protein samples. Methyl-beta-cyclodextrin (MBCD) can efficiently capture a wide variety of detergents commonly used for the stabilization of membrane proteins by sequestering detergent monomers. Reaction Center (RC...