Fluorescent proteins serve as important biomarkers for visualizing both subcellular organelles in living cells and structural and functional details in large-volume tissues or organs. However, current techniques for plastic embedding are limited in their ability to preserve fluorescence while remaining suitable for micro-optical sectioning tomography of large-volume samples. In this study, we q...

4-Hydroxyanisole (4-OHA) was administered to C57Bl/lOJ mice in which B16 melanoma or Lewis lung carcinoma had been implanted s.c. or i.m. The drug had the largest antitumour effect against B16 melanoma growing s.c. and a smaller antitumour effect against B16 melanoma or Lewis lung carcinoma growing i.m. In the treatment regimens where drug was administered only after tumour implantation, a sign...

The selenium analogues of three known inhibitors of chemical carcinogenesis were synthesized and the compounds were tested for their ability to inhibit the induction of forestomach tumors in mice by benzo(a)pyrene. Groups of female CD-1 mice were given NIH-07 diet, or NIH-07 diet to which one of the following test compounds had been added: p-methoxyphenol (30 mumol/g diet and 3.3 mumol/g diet);...

Saturation mutagenesis was used to generate 10 mutants of toluene-o-xylene monooxygenase (ToMO) at alpha subunit (TouA) positions A101 and A110: A101G, A101I, A101M, A101VE, A101V, A110G, A110C, A110S, A110P, and A110T; by testing the substrates toluene, o-cresol, m-cresol, p-cresol, phenol, naphthalene, o-methoxyphenol, m-methoxyphenol, p-methoxyphenol, o-xylene, and nitrobenzene, these positi...

Total synthesis of (±)-antroquinonol D, which is isolated from very expensive and rarely found Antrodia camphorata and which has potential anticancer properties, was achieved from 4-methoxyphenol. In addition, a Michael addition to dimethoxy cyclohexadienones was studied. The main step involved chelation and substrate-controlled diastereoselective reduction of cyclohexenone and lactonization. L...

The effects of feeding p-methoxyphenol, benzyl isothiocyanate, coumarin, alpha-angelicalactone, 2-tert-butyl-4-hydroxyanisole, and 3-tert-butyl-4-hydroxyanisole on the glutathione S-transferase activity and sulfhydryl levels of esophagus and small bowel mucosa of ICR/Ha mice have been investigated. p-Methoxyphenol, benzyl isothiocyanate, coumarin, and 2-tert-butyl-4-hydroxyanisole increased glu...

To analyze the role of cell proliferation in phenolic compound-induced rat forestomach carcinogenesis, early forestomach histopathological changes as well as oncogene expression and reversibility of early forestomach lesions were examined in F344 male rats. For the analysis of early lesions, five animals each were treated with butylated hydroxyanisole (BHA), caffeic acid, sesamol, or 4-methoxyp...

The volatile constituents of taheebo (the dried inner bark of Tabebuia impetiginosa Martius ex DC) were isolated by three methods: solvent-assisted flavour evaporation (SAFE); steam distillation under reduced pressure, followed by continuous liquid–liquid extraction (DRP-LLE); and high-flow dynamic headspace sampling (including closed-loop stripping) (DHS). The extracts were analysed qualitativ...

-Six phenols [2(3)-t-butyl-4-hydroxyanisole (BHA), 2-t-butylphenol, 4-methoxyphenol, 4-methylmercaptophenol, t-butylhydroquinone and 2,6-di-tbutylphenol] previously shown to be inhibitors of benzo(a)pyrene-induced neoplasia, were examined for their ability to induce in vivo changes in hepatic mono-oxygenase and detoxication enzyme activities, and to act as mono-oxygenase inhibitors when added i...

Compoundswith two phenolic OH groups like curcumin possess efficient antioxidant and anti-inflammatory activity. We synthesized p-cresol dimer (2,2'-dihydroxy-5,5'-dimethylbiphenol, 2a) and p-methoxyphenol dimer (2,2'-dihydroxy-5,5'-dimethoxybiphenol, 2b) by ortho-ortho coupling reactions of the parent monomers, p-cresol (1a) and p-methoxyphenol (1b), respectively. Their antioxidant activity wa...