By isolation of a mixed disulfide product of glutathione and cysteine, glutathione peroxidase was shown to be highly specific for only one donor substrate. Using the coupled assay of NADPH and yeast glutatione reductase, which is highly specific for flutathione disulfide, it was shown that the apparent inhibition of glutathione peroxidase by mercaptoethanol can be described kinetically and that...

Mild detergent treatment (0.1% Sarkosyl-0.1% beta-mercaptoethanol) of Dane particle-rich fraction from human serum resulted in the release of core particles together with HBe antigen activity when examined by the reversed passive haemagglutination method. Furthermore, when the core particles isolated by the above procedure were exposed to stronger detergent (1% Sarkosyl-0.1% beta-mercaptoethano...

Disullide bonds in proteins and in seed meals were reduced with /3-mercaptoethanol to sulfhydryl groups. The generated sulfhydryl groups were then alkylated by treatment with 4-vinylpyridine. The cysteine residues were thus derivatized to S-(4-pyridylethyl)-L-cysteine residues. The cysteine derivative is stable to acid hydrolysis and on amino acid analysis by ion exchange elutes just before arg...

We have shown in previous reports that cooked sorghum protein is less digestible than other cooked cereal proteins. The pepsin-indigestible proteins in sorghum were found to be mainly prolamin proteins. Cooking sorghum in the presence of 2-mercaptoethanol increased protein digestibility (in vitro with pepsin or trypsin/chymotrypsin) to a level comparable with other cereals. At a concentration o...

Semisolid culture systems have been developed which permit the proliferation of mousehemopoietic progenitor cells to form colonies of neutrophils and macrophages (1, 2), erythropoietic cells (3), eosinophils (4), and megakaryocytes (5) . These cloning systems have made it possible to analyse in detail many aspects of the nature and control of these hemopoietic populations . With a cloning syste...

CLINICAL CHEMISTRY, Vol. 23, No. 2, 1977 305 captoethanol was used per liter. We consider that this is due to the known tendency of mercaptoethanol to form aggregates with immunoglobulins, mainly IgG3 and 1gM (3-5). This observation was confirmed when we investigated the effect of two concentrations of mercaptoethanol (2.5 X i0 and 4.0 X 10 mol/liter) on the serum proteins, including immunoglob...

It is now commonly accepted that thiol activated creatine kinase (CK) assay systems measure CK activity more accurately and more reproducibly than non-activated systems. However, some differences have arisen in the literature in regards to the nature and course of CK activation. Some thiol compounds have been reported to yield higher enzyme activities than others. Dalal et al. (1) reported that...