macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...

Two distinct forms of globin messenger RNA were isolated from mouse spleen cells infected with Friend erythroleukemia virus: polyribosomal messenger ribonucleoprotein particles (15S mRNP), and their corresponding protein-free mRNAs obtained by chemical deproteinization. The translation efficiencies of both messenger forms were assayed in a Krebs II ascites cell-free system. Selective removal of...

The translations of native messenger RNA for rabbit globin and that of poly(A)-free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A)-free mRNA is close to that found with intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A)-free mRNA is considerably lower than with native mRNA. Simil...

Activation of pre-messenger RNA (pre-mRNA) splicing requires 5' splice site recognition by U1 small nuclear RNA (snRNA), which is replaced by U5 and U6 snRNA. Here we use crosslinking to investigate snRNA interactions with the 5' exon adjacent to the 5' splice site, prior to the first step of splicing. U1 snRNA was found to interact with four different 5' exon positions using one specific seque...

objective: the lrr (leucine rich proteoglycans) is a molecular recognition motif found in proteins with some roles in cell adhesion, signal transduction, dna repair and rna processing. opticin is a member of this family. takanosu et al (2001) detected messenger rna expression of mouse opticin in the eye, heart, brain, testis, thyroid and epididymis by dot blot hybridization. in this study, exp...

Experimental studies on mRNA stability have established several, qualitatively distinct decay patterns for the amount of mRNA within the living cell. Furthermore, a variety of different and complex biochemical pathways for mRNA degradation have been identified. The central aim of this paper is to bring together both the experimental evidence about the decay patterns and the biochemical knowledg...

Therapeutics based on messenger RNA (mRNA) have become a promising class of methods for various diseases’ treatments in recent years. The safe and effective delivery mRNA into target cells is the premise clinical translation mRNA. Due to protonation ionizable lipids acidic conditions, lipid nanoparticles (iLNPs) lower toxicity higher endosomal escape efficiency than permanent cationic (cLNPs). ...

The messenger RNA for chicken serum albumin together with its specific binding proteins was purified by hybrid selection using filter-bound cloned albumin cDNA. Under conditions where hybridization of the protein-mRNA complex was specific for the immobilized cDNA sequence, no dissociation of the complex into its protein and RNA components was apparent. Electrophoretic analysis of albumin mRNA-s...

Estrous cycle is one of the placental mammal characteristics after sexual maturity, including estrus stage (ES) and diestrus (DS). important in female physiology its disorder may lead to diseases, such as polycystic ovary syndrome, ovarian carcinoma, anxiety, epilepsy. In latest years, effects non-coding RNAs messenger RNA (mRNA) on estrous have started arouse much concern, however, a whole tra...

Abstract The 2022 Science for the Future Solvay Prize has been awarded to Katalin Karikó, adjunct professor at University of Pennsylvania (USA) and Szeged (Hungary), her work on biochemical modification synthetically produced messenger RNA (mRNA), which enabled rapid development vaccines. Her research was most notably used by Pfizer/BioNTech Moderna build COVID-19 mRNA vaccines, have saved many...