A rapid, sensitive and reproducible HPLC method was developed and validatedfor the analysis of amifostine (AMF) and/or its metabolite, WR-1065 inhuman plasma. The method involves the alkylation of free sulfydryl group with iodoacetic acid followed by derivatization of the drug and its metabolite witho-phthaldialdehyde (OPA) andUVdetection at 340 nm. The derivatized AMF and WR-1065 were eluted i...

a rapid, sensitive and reproducible hplc method was developed and validatedfor the analysis of amifostine (amf) and/or its metabolite, wr-1065 inhuman plasma. the method involves the alkylation of free sulfydryl group with iodoacetic acid followed by derivatization of the drug and its metabolite witho-phthaldialdehyde (opa) anduvdetection at 340 nm. the derivatized amf and wr-1065 were eluted i...

imatinib is an orally administered tyrosine kinase inhibitor which inhibits the bcr-abl protein-tyrosine kinase with high selectivity. imatinib is rapidly absorbed from the gut, after oral intake and has an almost absolute bioavailability of 98%. the metabolism of imatinib is mediated by the cytochrome p450 (cyp) isoenzymes in the liver and gut wall. cgp74588 is a major active metabolite of ima...

Background: The majority of studies on soil Aspergillus concern the isolation and characterization of the antimicrobial compounds produced by this organism. Our previous studies indicated an isolated Aspergillus strain soil to be of interest, and this subject is further investigated here. Method:  Soil samples of various locations in Iran were collected. Extract from Aspergillus sp. culture was...

a bioequivalence study of two verapamil formulations (generic verapamil tablets and isoptin® tablets) was performed by comparing pharmacokinetic parameters of the parent drug and its major metabolite, norverapamil following a single dose administration of 80 mg verapamil hydrochloride in 22 healthy volunteers according to a randomized, two-period, crossover-design study. moreover, the feasibili...

Protein–metabolite interactions play an important role in the cell’s metabolism and many methods have been developed to screen them vitro . However, few can be applied at a large scale not alter biological state. Here we describe proteometabolomic approach, using chromatography generate cell fractions which are then analyzed with mass spectrometry for both protein metabolite identification. Int...

diagnosing inherited metabolic disorders is a joint effort of both clinical and laboratory disciplines. various aspects of metabolite, enzyme and mutation analysis will be discussed based on the experience of the metabolic section of the erasmus university medical centre. where should laboratory diagnosis start from? metabolite, enzyme or dna level? the emphasis will be on enzyme analysis of th...