5-Fluoro-2'-deoxyuridylate causes a rapid inactivation of thymidylate synthetase that is dependent upon prior complexation of the cofactor 5,10-methylenetetrahydrofolate. The enzyme-5-fluoro-2'-deoxyuridylate complex may be isolated on nitrocellulose membranes and is not disrupted by 6 M urea. Upon reaction of 5-fluoro-2'-deoxyuridylate with the enzyme in the presence of 5,10-methylenetetrahydr...

AIM One of the main problems concerning repeated spontaneous miscarriage (RSM) is the etiological diagnosis. The relation of thrombophilia to RSM is a matter of debate. In this case-control study, we determined the percentages of three thrombophilic mutations (factor V leiden, prothrombin, and methylenetetrahydrofolate reductase) amongst 20 cases with RSM and 20 control normal parous women. R...

introduction: one factor known to cause thrombophilia in women with unexplained recurrent spontaneous abortion (rsa) is c677t polymorphism of methylenetetrahydrofolate reductase gene. this study aimed to determine the association between rsa and mthfr c677t polymorphism in iranian patients. methods: in this case-control study, 30 patients with previous history of two or more consecutive unexpla...

background: inherited thrombophilic gene polymorphisms have been related to the pathogenesis of venous thromboembolism and its outcomes. considering the scarcity of data on the frequency of the thrombophilic gene polymorphisms in iranian populations, the aim of this study was to assess such polymorphisms in healthy individuals. materials and methods: this cross-sectional study was performed on ...

A direct spectrophotometric method has been devised to follow the oxidation of tetrahydrofolate during the synthesis of thymidylate. It is based on the marked spectral change which occurs when 5, IO-methylenetetrahydrofolate is converted to dihydrofolate. A nonenzymatic reaction between formaldehyde and tetrahydrofolate yields 5,lO -methylenetetrahydrofolate (also called hydroxymethyltetrahydro...

important genetic-environmental interaction. Blood 91:4158, 1998 2. Jacques PF, Bostom AG, Williams RR, Ellison RC, Eckfeldt JH, Rosenberg IH, Selhub J, Rozen R: Relation between folate status, a common mutation in methylenetetrahydrofolate reductase, and plasma homocysteine concentrations. Circulation 93:7, 1996 3. Verhoef P, Kok FJ, Kluijtmans LAJ, Blom HJ, Refsum H, Ueland PM, Kruyssen DACM:...

important genetic-environmental interaction. Blood 91:4158, 1998 2. Jacques PF, Bostom AG, Williams RR, Ellison RC, Eckfeldt JH, Rosenberg IH, Selhub J, Rozen R: Relation between folate status, a common mutation in methylenetetrahydrofolate reductase, and plasma homocysteine concentrations. Circulation 93:7, 1996 3. Verhoef P, Kok FJ, Kluijtmans LAJ, Blom HJ, Refsum H, Ueland PM, Kruyssen DACM:...

BACKGROUND AND OBJECTIVES Intrauterine growth restriction is an important cause of morbidity and mortality. Its pathogenesis is still a matter of debate. The aim of this study was to evaluate the association between intrauterine growth restriction (diagnosed in utero by serial ultrasound examinations and characterized by abnormal umbilical arterial Doppler velocimetry) and thrombophilic polymor...