Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans. It is a well known cause of bovine reproductive losses such as abortion, infertility, stillbirth and birth of weak calves. Inthis research, the relationship between the seroprevalence rate of Leptospira spp. infection and abortion inindustrial dairy farms of Hamedan province, Iran was studied. A total of 80...

A microagglutination method utilizing stained antigen for detecting and measuring serum agglutinins against Francisella tularensis is described. The microagglutination and standard tube agglutination techniques were demonstrated to be comparable in sensitivity and specificity. Advantages of the micro method are rapidity and ease of performance, economy of reagents and, in particular, ease of in...

The sensitivity of an indirect immunofluorescence antibody test (IFAT) and a rapid microagglutination test (RMAT) for the diagnosis of Legionnaires' disease caused by Legionella pneumophila serogroup 1 was evaluated using serum specimens from 119 patients with bacteriologically confirmed infections. The sensitivity of both assays was found to be about 80%. In addition, antibody titres suggestiv...

Two identical leptospiral isolates coded Sh9 and Sh25 obtained from the urine of captive African giant pouched rats (Cricetomys gambianus), destined for use as biodetector of antipersonnel landmines were typed as serovar Kenya using cross-agglutination absorption test and DNA fingerprinting with the insertion element sequences IS1533 and IS1500 derived primers. The two isolates were previously ...

The presence of antibodies was determined in the serum and cerebrospinal fluid in six patients with neurobrucellosis using the Rose Bengal test, the microdilution agglutination test, and the Coombs' test. Four of the patients were followed up for more than three months. The Rose Bengal test and the microagglutination test were positive in cerebrospinal fluid in five of the six cases at some sta...

A solid-phase radioimmunoassay was developed for measuring the concentration of antispermatozoa antibodies in the sera of sterile patients. Whole human spermatozoa fixed in methanol and immobilized on plastic microtiter plates were used as antigens, and the spermatozoa-bound antibodies were quantitated with the aid of [125I]protein-A. The assay is specific, simple to perform and up to 1000 test...