In this study, vanillin, a quorum sensing (QS) blocker, and proteinase K were employed to investigate the effect of QS and extracellular proteins on the microbial attachment of aerobic granular activated sludge (AGAS). Results clearly showed that both vanillin and proteinase K could reduce attachment potential of AGAS, and the combined use of them was more effective in reducing attachment bioma...

Detailed characterization of post-translational modifications (PTMs) of proteins in microbial communities remains a significant challenge. Here we directly identify and quantify a broad range of PTMs (hydroxylation, methylation, citrullination, acetylation, phosphorylation, methylthiolation, S-nitrosylation and nitration) in a natural microbial community from an acid mine drainage site. Approxi...

Mass spectrometry (MS) has greatly enhanced research in the field of microbial proteomics. Microbial characterization, including detection, differentiation, and identification, are the most important tasks of microbial proteomic laboratories worldwide.2-DE is a well-established technique for separation and quantitation of a large set of proteins and has much to contribute to the experimental an...

The chicken gastrointestinal tract (GIT) harbours a complex microbial community, involved in several physiological processes such as host immunomodulation and feed digestion. For the first time, the present study analysed dietary effects on the protein inventory of the microbiome in crop and ceca of broilers. We performed quantitative label-free metaproteomics by using 1-D-gel electrophoresis c...

background:isolates of staphylococcus aureus express a myriad of adhesive surface proteins that play important role in colonization of the bacteria on nasal and skin surfaces, beginning the process of pathogenesis. the aim of this study was to screen several of the microbial surface components recognizing adhesive matrix molecules (mscramms) genes among the isolate of s. aureus from hospitalize...