CONTEXT Cytological assessment of pancreatic juice is commonly used to diagnose pancreatic ductal adenocarcinoma; however, the sensitivity of cytological assessment has been reported to be low. MicroRNAs are small RNAs regulating various cellular processes and have recently been identified as possible markers of malignant diseases including pancreatic ductal adenocarcinoma. OBJECTIVE The purp...

A novel signal "on" type of photoelectrochemical biosensor for microRNA-21 hybridization detection was fabricated, where Bi2S3 nanorods were used as photoactive material with a maximum adsorption at 450 nm visible light, hairpin-structure DNA as detecting probe, streptavidin as signal capturing unit and biotin functionalized ascorbic acid loaded apoferritin as signal amplification unit. Hybridi...

BACKGROUND Pulmonary hypertension (PH) is driven by diverse pathogenic etiologies. Owing to their pleiotropic actions, microRNA molecules are potential candidates for coordinated regulation of these disease stimuli. METHODS AND RESULTS Using a network biology approach, we identify microRNA associated with multiple pathogenic pathways central to PH. Specifically, microRNA-21 (miR-21) is predic...

We report here an impedimetric genosensor for the quantification of microRNA-21 using [Fe(CN)6]3?/4? as redox probe to transduce hybridization event. The biosensing platform was built at a thiolated-gold electrode by covalent bond reduced graphene oxide (RGO) modified with chitosan (CHIT) and further attachment aminated DNA probe. GO used provide carboxylic groups CHIT and, once reduced, improv...

MicroRNA are small noncoding RNA molecules that regulate gene expression. To investigate the role of microRNA in immune thrombocytopenia (ITP), we performed genome-wide expression analyses of mRNA and microRNA in T cells from ITP patients and controls. We identified 1915 regulated genes and 22 regulated microRNA that differed between ITP patients and controls. Seventeen of the 22 regulated micr...

TGF-β(1) is a pleotropic growth factor that mediates glomerulosclerosis and podocyte apoptosis, hallmarks of glomerular diseases. The expression of microRNA-21 (miR-21) is regulated by TGF-β(1), and miR-21 inhibits apoptosis in cancer cells. TGF-β(1)-transgenic mice exhibit accelerated podocyte loss and glomerulosclerosis. We determined that miR-21 expression increases rapidly in cultured murin...

BACKGROUND MicroRNA expression is severely disrupted in carcinogenesis, however limited evidence is available validating results from cell-line models in human clinical cancer specimens. MicroRNA-21 (mir-21) and microRNA-143 (mir-143) have previously been identified as significantly deregulated in a range of cancers including cervical cancer. Our goal was to investigate the expression patterns ...