genetic diversity of red jabal barez red goat population was studied based on eight microsatellite markers consist bm1312, maf64, ilsts034, ilsts059, orafcb20, il2ra, lscv24 and lscv11. blood sample extracted of 100 red jabal barez goats were randomly collected from 10 farms and dna was isolated using the diatom dna prep kit. the pcr reactions were successfully done and all loci showed suitable...

Vol. 36, No. 3 (2004) BioTechniques 409 The process of microsatellite development and profiling involves three primary steps: (i) isolating regions of genomic DNA that contain microsatellite loci; (ii) developing strategies for screening each locus, which requires designing primers for amplification, optimizing reaction conditions, and screening for variation; and (iii) genotyping sampled indiv...

this study investigated genetic variation within indigenous chicken of khorasan using four microsatellite markers (mcw5, mcw16, mcw18 and mcw34). 90 blood samples were collected from this population and phenol-chloroform extraction method was used to isolate the dna. the highest and the lowest allele number and effective alleles are shown in mcw5 (6 and 5.293) and mcw34 (4 and 3.138), respectiv...

This study was conducted in order to investigate genetic diversity and population structure of pike-perch in the Northern part of Iran. For this purpose, 207 adult pike-perches from four regions of the Caspian Sea watershed (Talesh Coasts, Anzali Wetland, Chaboksar Coasts and Aras Dam) were collected. DNA was extracted and by using 15 pairs of microsatellite primers, Polymerase Chain Reaction (...

Microsatellite alterations within genomic DNA frameshift as a result of defective DNA mismatch repair (MMR). About 15% of sporadic colorectal cancers (CRCs) manifest hypermethylation of the DNA MMR gene MLH1, resulting in mono- and di-nucleotide frameshifts to classify it as microsatellite instability-high (MSI-H) and hypermutated, and due to frameshifts at coding microsatellites generating neo...

background: retinoblastoma is the most common intraocular tumor in childhood and mutation in the rb1 gene will trigger the tumorigenesis. so far, a wide range of the mutations along the length of rb1 gene have been reported. however, some could not be detected by common detection methods. in such condition, linkage analysis using microsatellite markers is suggested to trace unknown rb1 mutation...

Understanding the scale at which wild stocks of Persian sturgeon (Acipenser persicus) are genetically discrete is necessary for effective management of this commercially important species. Disomic DNA microsatellite markers are among the best tools for determining stock structure in fishes. As all sturgeon species have a polyploid ancestry of all sturgeons, most gene loci exhibit more than two ...

BACKGROUND Organ-confined renal malignancies can be cured in the majority of patients, whereas more extensive lesions have a poor prognosis. We sought to develop a noninvasive test for renal cancer detection based on a novel molecular approach. METHODS Matched urine and serum DNA samples were obtained before surgery from 30 patients with clinically organ-confined solid renal masses (25 with m...

A procedure for selection of wheat genomic fragments containing various microsatellites is described. The procedure is based on the hybridization of single-stranded DNA fragments either to filter bound microsatellite DNA or to biotinylated oligonucleotides in solution. Hybridized strands are isolated, amplified and cloned. Results presented show that enrichment for (CA)n/(TG)n microsatellites d...

A series of techniques are presented to construct genomic DNA libraries highly enriched for microsatellite DNA loci. The individual techniques used here derive from several published protocols but have been optimized and tested in our research laboratories as well as in classroom settings at the University of South Carolina and University of Georgia, with students achieving nearly 100% success....