Ruminal fermentation products such as short-chain fatty acids (SCFA) and CO2 acutely stimulate urea transport across the ruminal epithelium in vivo, whereas ammonia has inhibitory effects. Uptake and signaling pathways remain obscure. The ruminal expression of SLC14a1 (UT-B) was studied using polymerase chain reaction (PCR). The functional short-term effects of ammonia on cytosolic pH (pHi) and...

This experiment investigated the effect of dietary crude protein (CP) and ruminally degraded protein (RDP) levels on rumen fermentation, digestibility, ammonia emission from manure, and performance of lactating dairy cows. The experiment was a replicated 3 x 3 Latin square design with 6 cows. Three diets varying in CP concentration were tested (CP, % of dry matter): 15.4 (high CP, control), 13....

Two experiments were conducted to evaluate effects of barley grains particle size on ruminal pH and ammonia concentration of rams (experiment 1) and carcass characteristics (experiment 2) of male lambs fed high urea diet. Treatments in two experiments were (1: basal diet + whole barley grains, 2: basal diet + ground barley grains with a 5 mm screen, 3: basal diet + ground barley grains with a 3...

Four multiparous lactating Holstein cows fitted with rumen cannulae were fed diets varying in the amount and source of rumen-degradable carbohydrates (starch vs. sucrose) to examine their effects on rumen fermentation, nitrogen metabolism and lactation performance. A 4×4 Latin square with four diets and four periods of 28 days each was employed. Corn starch and sucrose were added to diets and c...

to examine the effects of different sources of rumen-degradable carbohydrates on rumen fermentation, nitrogen metabolism and performance of holstein dairy cows, four lactating holstein cows fitted with rumen cannulae in a 4×4 latin square with four diets and four 28 day periods each, were employed. experimental diets contained different levels of pure sucrose and corn starch in which sucrose wa...

Blood and ruminal samples were analyzed from gestating cows fed apple pomace or corn silage plus isonitrogenous and isocaloric quantities of cottonseed meal or nonprotein nitrogen containing supplements in four trials. Data were also obtained from steers fed similar diets during a metabolism trial. The supplementary sources of nitrogen supplied 50 to 70% of the total nitrogen in the diet. Feedi...

Four experiments were conducted to investigate the effects of ractopamine hydrochloride (RAC) on ruminal fermentation and proteolysis. In Exp. 1, in vitro gas and VFA production was measured in flasks incubated with 0, 0.226, 2.26, 22.6, and 226.0 mg of RAC/L of buffered ruminal fluid. Ractopamine hydrochloride had a quadratic effect on in vitro gas production (P < 0.05; 177, 181, 185, 190, and...

three multiparoys lactating sannen dairy goats in midlactation with mean milk production and dim pf 1.5 ±0.22 kg and 167±4 days were used to determine effects of the abomasal infusion wheat starch, wheat starch or cottonseed oil with casein on performance of sannen dairy goats. goats were fed ad libitum with basal diet include, 40% hay alfalfa and 60% concentrate. treatments were abomasal infus...

The objective of this study was to determine the effects of diets having different fermentability on blood gases, blood metabolites and ruminal fermentation parameters in Holstein dairy cows. Four multiparous Holstein dairy cows (665 ± 25 kg BW, 170 ± 7.0 DIM and 15±1.5 kg of milk yield), fitted with rumen cannulae, were used in a 4×4 Latin Square design with 28-d periods. Corn starch and sucro...

A laboratory silo trial, two lamb metabolism trials and a lamb growth trial were conducted to compare the nitrogen (N) value of swine methane digester effluent (MDE) to that of urea. Using laboratory silos (four/treatment), fermentation characteristics were measured for silages containing cracked corn, ground wheat straw and MDE added at levels of 0, 4, 8 or 12% of silage dry matter (DM). Lacti...