The continuous increase of available biological data as consequence of modern high-throughput technologies poses new challenges for analysis techniques and database applications. Especially for miRNAs, one class of small non-coding RNAs, many algorithms have been developed to predict new candidates from next-generation sequencing data. While the amount of publications describing novel miRNA can...

The miRBase is the official miRNA repository which keeps the annotation updated on newly discovered miRNAs: it is also used as a reference for the design of miRNA profiling platforms. Nomenclature ambiguities generated by loosely updated platforms and design errors lead to incompatibilities among platforms, even from the same vendor. Published miRNA lists are thus generated with different profi...

Hepatitis C virus (HCV) infection causes acute and chronic hepatitis leading to cirrhosis and hepatocellular carcinoma which are the major health problems around the world including Thai population. Many recent studies were shown an important role of microRNA (miRNA) to inhibit or promote viral replication. This study aimed to investigate human miRNAs and analyze hybridization patterns between ...

Introduction: The Epstein–Barr virus (EBV) W repeats are transcribed during viral lytic reactivation, a highly oncogenic form of latency known as latency III, and a rare type of latency (Wp-restricted) that is observed in ~15% of endemic Burkitt’s lymphomas. The W repeats encode an EBV protein, EBNA-LP, whose message is produced by splicing out a short (81 nt) and long (2791 nt) intron. We prev...

BACKGROUND Micro(mi)RNAs are short RNA sequences, ranging from 16 to 35 nucleotides (miRBase; http://www.mirbase.org). The majority of the identified sequences are 21 or 22 nucleotides in length. Despite the range of sequence lengths for different miRNAs, individual miRNAs were thought to have a specific sequence of a particular length. A recent report describing a longer variant of a previousl...

MicroRNAs (miRNAs) are a class of non-coding RNAs that regulate gene expression. Identification of total number of miRNAs even in completely sequenced organisms is still an open problem. However, researchers have been using techniques that can predict limited number of miRNA in an organism. In this paper, we have used homology based approach for comparative analysis of miRNA of hexapoda group ....

MicroRNAs (miRNAs) have been demonstrated to be potent post-trascriptional modulators of protein expression. miRNA expression was profiled in the left and right dorsal hippocampal CA3 of mature rats by high-throughput deep sequencing. Among the sequenced and cross-mapped small RNAs, 88% belonged to the miRNAs annotated in the miRBase 15 database. Nearly half of the small RNAs belonged to the le...

MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. Version 20 of the miRNA registry miRBase contains 307 mature miRNAs and 200 pr...

We thank Backes and Keller (1) for their comments on our recent publication (2). With regard to the first example in their cluster analysis point, it is great to see that Backes and Keller (1) uncovered more clusters among our reported data, as this provides further support for the nonaccidental nature of these molecules. With regard to the second example, where they did not find a direct overl...