Synthesis of nuclear and cytoplasmic proteins was studied biochemically and autoradiographically in early loach (Misgurnus fossilis) and sea-urchin (Strongylocentrotus nudus) embryos. After incubation with [C]amino acids for 5-120 min the ratio of the specific activities of nuclear, mitochondrial and 12000g supernatant proteins was shown to be equal approximately to 6:1:2 in loach embryos and t...

Two distinct cDNAs encoding aquaporins (mmAQPs 1a and 3a) were isolated and characterized from mud loach Misgurnus mizolepis. The identified mud loach AQP cDNAs encode for polypeptides of 260 and 302 amino acids. Topology predictions confirmed six putative membrane-spanning domains connected by five loops and the Nand C-terminal domains being cytoplasmic. The mud loach AQPs 1a and 3a showed bro...

An animal lectin purified from loach (Misgurnus anguillicaudatus) eggs induced release of cytotoxin from fresh bone marrow cells from mice, although the other lectins tested, wheat germ agglutinin, concanavalin A, and phytohemagglutinin did not. The cytotoxin released from bone marrow cells was a heat-labile protein with a molecular weight of 70,000. The main cells responsible for release of Mr...

Experiments on the capacity of the isolated teleost blastoderm to undergo development when cultivated in vitro have revealed species differences in the time of initiation of blastoderm differentiation. Thus the Fundulus blastoderm acquires the ability to develop after being separated from the yolk at the stage of 32 blastomeres (Oppenheimer, 1936) while the blastoderms of pike and of salmon dif...

The present study was aimed to investigate the effects of microcystin–LR (MCLR) on the blood electrolytes of Heteropneustes fossilis. The fish were injected microcystinLR (2.5 μg/25 g) intraperitoneally at the initiation of the experiment. Blood samples were collected and the serum calcium and phosphate levels were estimated at 1, 3, 5, 10, 15 days. There is no change in serum calcium level of ...