Phylogenetic relationships and genetic variation between two Iranian sheep breeds were analyzed using cytochrome b (cyt-b) gene sequences. The genomic DNA was isolated by salting out method and amplified cytochrome b gene using polymerase chain reaction restriction (PCR) method with a pair of primer. A partial sequence of cyt-b gene of Iranian sheep is 780 bp and contained 13 variable sites and...

Direct sequencing of mitochondrial DNA (mtDNA) following amplification using the polymerase chain reaction (PCR) has found widespread use in population genetic and phylogenetic research over the past few years. Recently, nuclear copies of mitochondrial genes have been reported in diverse eukaryotic species, often confounding such research (reviewed in [2,3]). Under certain circumstances, nuclea...

Rodents from the subfamily Gerbillinae Gray, 1825, are an important component of small fauna in arid and desertic regions Africa Asia. Among this group rodents, Sundevall’s jird, Meriones crassus, is one most widespread desert species, found countries ranging Morocco to Pakistan. In study, we sequenced cytochrome b gene mitochondrial DNA two specimens captured Morocco, constituting first geneti...

We assessed the mitochondrial DNA sequence divergence of a 718 bp fragment of the control region and 1007 bp of the cytochrome b gene between two allopatric morphologically different subspecies of bluethroat (Luscinia svecica). None of the 17 total haplotypes was shared between L. s. namnetum and L. s. svecica. However, the mean distances between subspecies were very low for both fragments (0.0...

We have previously used inhibitors interacting with the Qn site of the yeast cytochrome bc(1) complex to obtain yeast strains with resistance-conferring mutations in cytochrome b as a means to investigate the effects of amino acid substitutions on Qn site enzymatic activity [M.G. Ding, J.-P. di Rago, B.L. Trumpower, Investigating the Qn site of the cytochrome bc1 complex in Saccharomyces cerevi...

the identification of the efficiency of some mtdna genes of mus musculus species complex (house mouse) for biosystematics research was studied in this approach. recent studies have made use of different mitochondrial genes including nadh dehydrogenase genes, cytochrome b gene, cytochrome oxidase genes, d-loop region and whole mtdna genome to study the house mouse species. usage of each of these...

Mice homozygous for a defect in the PTCD2 (pentatricopeptide repeat domain protein 2) gene were generated in order to study the role of this protein in mitochondrial RNA metabolism. These mice displayed specific but variable reduction of ubiquinol-cytochrome c reductase complex activity in mitochondria of heart, liver and skeletal muscle due to a decrease in the expression of mitochondrial DNA-...

We describe the development and validation of a qPCR assay to detect Poecilia reticulata, highly invasive species freshwater fish Caribbean islands, through environmental DNA (eDNA) sampling. Originating from Trinidad, this is detrimental countless native tropical communities. A assay, consisting set primers fluorescent probe, amplifying 214 base pair target region mitochondrial Cytochrome B ge...

Abstract The East European vole Microtus mystacinus is the most widespread species in Anatolia. It also frequently seen watery habitats a large area of Eurasia. In this paper, an attempt was made to ascertain level genetic differentiation between Anatolian (Asian part Turkey) and Turkish Thracian (European populations together with additional data from other parts Asia Europe by analysing two m...

background: haematophagous females of some phlebotomine sandflies are the only natural vectors of leishmania species, the causative agents of leishmaniasis in many parts of the tropics and subtropics, including iran.  we report the presence of phlebotomus (larroussius) major and phlebotomus (adlerius) halepensis in tonekabon (ma­zanderan province) and phlebotomus (larroussius) tobbi in pakdasht...