AIMS To determine whether lactic acid bacteria (LAB) used in inoculants for silage can survive in rumen fluid (RF), and to identify those that survive best. METHODS AND RESULTS Twelve commercial silage inoculants were added at 107 CFU ml-1 to strained RF (SRF) taken from dairy cows, with and without 5 g l-1 glucose and incubated in vitro at 39 degrees C. Changes in pH, LAB numbers and ferment...

An immunoblot procedure for the strain-specific quantitative analysis of commercial Rhizobium inoculants was developed. The technique greatly reduced the time required for inoculant analysis. Correlation between immunoblot analysis and traditional plant nodule grow-out most-probable-number techniques was r = 0.90 for 16 commercial alfalfa inoculants tested.

Rhizosphere competence of bacterial inoculants is assumed to be important for successful biocontrol. Knowledge of factors influencing rhizosphere competence under field conditions is largely lacking. The present study is aimed to unravel the effects of soil types on the rhizosphere competence and biocontrol activity of the two inoculant strains Pseudomonas jessenii RU47 and Serratia plymuthica ...

The survival and effect of three new probiotic inoculants (Lactobacillus plantarum CCM 4000, L. fermentum LF2, and Enterococcus faecium CCM 4231) on the nutritive value and fermentation parameters of corn silage was studied under laboratory conditions. Whole corn plants (288.3 g/kg DM) were cut and ensiled at 21°C for 105 days. The inoculants were applied at a concentration of 1.0×10 cfu/ml. Un...

There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculants were used: Lactocacillus plantarum K KKP 593p (LPK), L. plantarum C KKP 788p (LPC), L. buchner...

Increased interests in low-input agriculture in recent years has seen the growing developmentin the use of commercial biological inoculants to increase the mobilization of key nutrients suchas nitrogen (N), phosphorus (P) and potassium (K) to enhance their availability to crop plants.The objectives of this field experiment with tobacco were to determine i) reduced rates ofinorganic fertilizer c...

in order to evaluate nitrogen fixation ability and nutrients uptake in three cultivars of common bean (phaseolus vulgaris l.) inoculating with some types of inoculants containing different strains of rhizobium leguminosarum; bv. phaseoli an experimental design was conducted in khoramdarah region in 2006 and 2007. in this investigation three types of biological fertilizers including rb117, rb123...

The long-term survival of Rhizobium phaseoli strains 127K17, 127K26, and 127K35 in legume inoculants prepared with eight different coals (one strain and one coal per inoculant) was studied. The coals used were Pennsylvania anthracite, bituminous coals from Illinois, Pennsylvania, and Utah, lignite from North Dakota and Texas, and subbituminous coals from New Mexico and Wyoming; they ranged in p...

Microorganisms are major key players for sustaining the soil quality degraded by intensive use of synthetic chemicals for increasing crop production and therefore, use of them as inoculants or biofertilizers is an integral part of sustainable agriculture. An effort was, therefore, made to examine the effect of cellulose degrading bacterial isolates on legume (Chickpea) based cropping systems. N...

Recent advances in silage microbiology are reviewed. Most new techniques in silage microbiology use the polymerase chain reaction (PCR) to make copies of a portion of the DNA in microorganisms. These techniques allow us to identify and quantify species as well as do community analysis. The PCR-based techniques are uncovering new species, both bacteria and fungi, during storage and feeding. Sila...