نتایج جستجو برای: molecular assay
تعداد نتایج: 833151 فیلتر نتایج به سال:
ABSTRACT Background and Objectives: Diagnosis of hepatitis E virus (HEV) infection could be missed in some cases if serological tests are used solely. Molecular characterization of HEV is essential for diagnosis of acute and chronic HEV infections, and evaluating the chronic HEV infection status in immunocompromised patients. T...
Molecular Identification of Listeria monocytogenes in Raw Hamburgers from Kerman, South-East of Iran
Background: Listeria monocytogenes is a Gram-positive and facultative anaerobic food-borne bacterium which is capable of intra and extra cellular growth. L. monocytogenes usually can exist on different surfaces and instruments at production and processing sites of food products with animal origin. In Iran, the consumption of burger has increased recently, but its safety is still of great concer...
Anticancer properties of chitosan against osteosarcoma, breast cancer and cervical cancer cell lines
Background: Cancer refers to the abnormal growth of cells and is still the most common cause of morbidity in world. The purpose of this study was to determine cytotoxicity effect of high molecular weight (HMWC) and low molecular weight of chitosan (LMWC) on three cancerous cell lines MCF-7, HeLa and Saos-2 with different histological origin. Methods: The anticancer property of two types of chi...
به دلیل عوارض غیر قابل جبران توکسوپلاسموزیس در افراد دچار نقص سیستم ایمنی و نوزادان متولد شده از مادران آلوده، نیاز مبرم به تکنیک های سریع، حساس و دقیق برای شناسایی توکسوپلاسما گوندی احساس می شود. روش های مولکولی به عنوان روش های حساس تر و اختصاصی-تر از رو ش های سرولوژیک در تشخیص توکسوپلاسموزیس مطرح هستند. در این مطالعه، استفاده از روش real-time nasba برای سنجش کمی بار انگلی در خون ر ت های آلو...
for the evaluation of autoantibodies in pemphigus vulgaris (pv), an indirect elisa assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (m.w.) of 130-160 kda. the evaluation of pemphigus igg, igm and iga autoantibodies in 75 patients and 50 control sera indicated that this indirect elisa assay was a useful method for the detection of ...
Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pterid...
background: classical bacteriological detection of brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. however, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. methods: panel of some reference strains belonging to t...
testosterone level was determined in six different populations of iran using saliva steroid assay. significant difference was observed in morning and afternoon testosterone level of the same individual (p>0.00l) significant difference was also noticed among different regions studied (p>0.00 1). comparisons between alcoholics and nonalcoholic as well as smokers and nonsmokers showed significant ...
Background: Evaluation of cell viability is momentous in pharmacologic and oncological research. Cell viability evaluation determines cell sensitivity and consequently treatment outcome. Various methods are available to determine cell survival. Each of these methods evaluates different endpoints. Accordingly, determining the correlation between these methods is important. In this study, in orde...
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