The main objective of this work was to explore the feasibility print monoclonal antibody (mAb)-loaded implantable systems using fused-deposition modelling (FDM) build complex dosage form designs. Indeed, our knowledge, is first investigation mAb-loaded devices FDM. To make possible, different steps were developed and optimized. A mAb solution stabilized trehalose (TRE), sucrose (SUC), hydroxypr...

Pancreatic cancer remains as one of the most aggressive types. In absence reliable biomarkers for its early detection and more effective therapeutic interventions, pancreatic is projected to become second leading cause death in Western world next decade. Therefore, it essential discover novel targets develop cancer-specific agents. To date, 45 monoclonal antibodies (mAbs) have been approved tre...

Abstract Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting receptor binding domain (RBD) of viral spike protein via antibody library constructed from peripheral blood mononuclear cells a convalescent patient. The CT-P59 mAb potently neutralizes SARS-Co...

Background: The B-cell antigen CD20 is expressed on normal B-cells and almost all lymphomas. This non-modulating agent provides an excellent target for antibody-directed therapeutic regimens. IDECC2B8 (rituximab) a monoclonal antibody (mAb) directed against the B-cell-specific CD20. mediates complement antibody-dependent cell-mediated cytotoxicity. As its usage has surged, there have been growi...

We present a numerical case study for modeling and simulation of upstream downstream processes monoclonal antibody (mAb) production. apply systematic intuitive methodology an existing process process. The resulting models are based on differential mass balances kinetic expressions the reactions adsorption. Mass fedbatch reactor yield model consisting five ordinary equations (ODEs). is conducted...

In silico frameworks have the potential to improve experimentally designed processes for monoclonal antibody (mAb) manufacturing. this paper, a framework analysis of mAb process alternatives is presented with objective realizing improvements from flowsheet perspective. The key elements proposed method include generation and decomposition design space, formulation model, simulation evaluation al...

We report the humanization and characterization of monoclonal antibody (MAb) T1-2 or tefibazumab, a monoclonal antibody that recognizes clumping factor A expressed on the surface of Staphylococcus aureus. We demonstrate that the binding kinetics of MAb T1-2 is indistinguishable compared to that of its murine parent. Furthermore, MAb T1-2 is shown to enhance the opsonophagocytic uptake of ClfA-c...

Rabies is an acute viral zoonotic disease that affects the central nervous system (CNS) of all warm-blooded animals, including mammals. Research studies and experience from across world have demonstrated appropriate administration a combination (a) local wound treatment, (b) anti-rabies vaccination (c) passive immunization proved to be quite effective in preventing occurrence rabies. As far as ...

Human plasma gelsolin was purified by column chromatography. The method yielded a protein of high purity and activity. Using this protein, we produced monoclonal antibody (Mab H6B11) against human plasma gelsolin by somatic cell fusion. This monoclonal antibody reacted in a dose-dependent manner with gelsolin derived from human plasma and platelets and neutralized depolymerizing activity to F-a...

Antibody-drug conjugates (ADCs) are biotherapeutics consisting of a tumor-targeting monoclonal antibody (mAb) linked covalently to cytotoxic drug. Early generation ADCs were predominantly obtained through non-selective conjugation methods based on lysine and cysteine residues, resulting in heterogeneous populations with varying drug-to-antibody ratios (DAR). Site-specific is one the current cha...