Jojoba (Simmondsia chinensis (Link) Schn.) is a nontraditional crop in arid and semi-arid areas. Vegetative propagation can be achieved by layering, grafting, or rooting semi-hardwood cuttings, but the highest number of possible propagules is limited by the size of the plants and time of the year. Micropropagation is highly recommended strategy for obtaining jojoba elite clones. For culture ini...

tissue culture methods provide tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, in vitro propagation of diaphanoptera khorasanica rech.f., a rare and threatened plant species with limited distribution range and population was investigated. this species has a potential as an ornamental plant. single node explants wer...

Micropropagation of Kainth using nodal explants was carried out in tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Per cent necrotic culture was found to be influenced by...

hyssop (hyssopus officinalis l.), belongs to the family of lamiaceae which its origin is asia minor, and is considered as one of the most important medicinal plant. this experiment was done to study the effects of population and bap concentrations on hyssop plant regeneration under in vitro culture conditions. this research were performed in factorial experiment with three populations (hamadan,...

This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting by different explants and different concentration of BAP. Due to optimization of shoot regeneration, two media including of MS and modified MS (MS salts with double concentration of CaC21, and B5 vitamins), different explants such as decapitated embryo axes, epicotyls and cotyledonary no...

in order to micropropagate of four vigorous rootstocks of pear, including pyrus betulifolia, konjuni, dargazi and gh1, effects of basic plant culture media (ms, ql and modified ql), cytokinins and auxins on productivity, proliferation and rooting were studied in seed and plant improvement institute, karaj. the highest amount of productivity for all rootstocks was observed in ql medium. among pe...

Nodal explants of Phyla nodiflora (L.) Greene, were cultured on Murashige and Skoog (MS) medium supplemented with different combination and concentration of cytokinins and auxins for multiple shoots regeneration. The maximum numbers of shoots were found in MS medium supplemented with2.5 mgl-16-benzylaminopurine (BA) and 0.5 mgl-1 kinetin (KN). Elongated shoots were transferred to rooting ...

gavane-gaz-angubin (persian manna) belongs to astragalus genus (leguminosae). this plant is one of the most important species in iran for its medicinal value. considering the low propagation rate of this plant from seed and cutting, the feasibility of in-vitro propagation of this plant was investigated. effect of different types and concentrations of cytokinins such as bap, kin, and ze (0.5, 1,...

Successful in vitro propagation through ex vitro rooting mechanism has been achieved in Piper longum by nodal shoot segment cultures. Shoot tip explants were less proliferative compared to the nodal meristems. The explants were sterilized using 0.1% HgCl2 and cultured on Murashige and Skoog (MS) medium with various concentrations of growth regulators. MS medium supplemented with 1.0 mg/L 6-benz...

Rhizomes of Smilax corbularia are used for the treatment of cancers, AIDS, septicemia and lymphatic diseases. The demand for rhizomes as an important ingredient in medicinal preparations has increased considerably. As rapid multiplication through in vitro propagation need to be developed, the effects of MS (Murashige and Skoog) medium salt strengths and plant growth regulators were investigated...