We have previously shown that cells with a high content of the gold-binding protein metallothionein (MT) are resistant to the growth inhibitory effects of gold(III) chloride and auranofin. To investigate whether MT confers resistance to sodium aurothiomalate two cell lines of cultured human epithelial (HE) cells were used; the parental cell line (HE) and a substrain (HE100) containing high leve...

Two antigen detection systems (MicroTrak [MT], Syva Co., Palo Alto, Calif.; and Chlamydiazyme [CZ], Abbott Laboratories, North Chicago, Ill.) were compared with semiquantitative culture for diagnosis of chlamydial infection in 1,059 patients. Cultures were done on microtiter plates and blind passaged once. Culture-negative but CZ- or MT-positive specimens were recultured. True positives were po...

The present study assessed the effect of resveratrol on the expression of SIRT1 and mitochondrial quality and quantity in porcine oocytes. Supplementing the maturation medium with 20 µM resveratrol increased the expression of SIRT1, and enhanced mitochondrial functions, as observed from the increased ATP content and mitochondrial membrane potential. Addition of resveratrol also improved the abi...

the major objective of seedless grape breeding program is production of grapes with high quality. breeding of seedless grapes is difficult because the embryos are aborted after fertilization. embryo rescue technique is utilized to obtain plants from crosses between seedless cultivars. in the present study, the effect of paternal genotypes and different iaa concentrations in presence of ba appli...

Murine Balb/3T3 and murine A-MT-BU-A1 mammary tumor cells were separated in the presence of cytochalasin B into enucleated cytoplasmic components (cytoplasts) and nucleated subcellular components (karyoplasts). Karyoplasts were derived from 3T3 cells, while cytoplasts were derived from A-MT-BU-A1 cells that were both chloramphenicol-resistant (CAP(r)) and sensitive to hypoxanthine/aminopterin/t...

We have recently shown, using antisense strategy, that the R11j3 regulatory subunit of cAMP-dependent protein kinase is essential for cAMP-induced growth inhibition and differentiation of HL-6O human leukemia cells. We constructed a retroviral vector for RII 3 (MT-RIIf ) by inserting human RII 3 complementary DNA into the OTi 521 retroviral vector plasmid that contains an internal mouse metallo...

background: in the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. a smaller medium drop can prevent dilution of this factor(s). objective: the objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. materials and methods: the em...

the germination power of orchids (orchidaceae family) seems to betoo weak due lack of albumen. the study carried out with various treatments including pollination time and ga3 for breaking dormancy and increasing seed germination of orchids. the effect of pollination time (8 periods from january to august) and gibberellic acid (0, 500, 1000 and 1500 mg l-1) were studied on germination of phalae...

A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involved the introduction of blood (and bacteria) into the growth medium with the dye...

We applied multiplex-tandem PCR (MT-PCR) to 255 EDTA whole-blood specimens, 29 serum specimens, and 24 plasma specimens from 109 patients with Candida bloodstream infection (candidemia) to determine whether a diagnosis could be expedited in comparison with the time to diagnosis by the use of standard blood culture. Overall, the MT-PCR performed better than blood culture with DNA extracted from ...