In most eukaryotic cells, microtubules (MTs) are assembled at identified nucleating sites, such as centrosomes or spindle pole bodies. Higher plant cells do not possess such centrosome-like structures. Thus, the fundamental issues of where and how the intracellular plant MTs are nucleated remain highly debatable. A large body of evidence indicates that plant MTs emerge from the nuclear peripher...

LIS1 was first identified as a gene mutated in human classical lissencephaly sequence. LIS1 is required for dynein activity, but the underlying mechanism is poorly understood. Here, we demonstrate that LIS1 suppresses the motility of cytoplasmic dynein on microtubules (MTs), whereas NDEL1 releases the blocking effect of LIS1 on cytoplasmic dynein. We demonstrate that LIS1, cytoplasmic dynein an...

An important consideration in developing standards and regulations that govern the production and use of commercial nanoscale materials is the development of robust and reliable measurements to monitor the potential adverse biological effects of such products. These measurements typically require cell-based and other biological assays that provide an assessment of the risks associated with the ...

Background. Human herpesvirus 6B (HHV-6B) is the causative agent for exanthema subitum. This virus has been suggested to have neurovirulence based on the in-vivo and in-vitro studies. HHV-6B has been suggested to be associated with mesial temporal sclerosis (MTS) that results in mesial temporal lobe epilepsy. Aims of this study was to elucidate pathogenic role of HHV-6B in patients with MTS. Me...

In most eukaryotic cells, microtubules (MTs) are assembled at identified nucleating sites, such as centrosomes or spindle pole bodies. Higher plant cells do not possess such centrosome-like structures. Thus, the fundamental issues of where and how the intracellular plant MTs are nucleated remain highly debatable. A large body of evidence indicates that plant MTs emerge from the nuclear peripher...

Introduction: Medial temporal sclerosis (MTS) is a form of mesial temporal lobe epilepsy (mTLE). It is typically characterized by structural alterations in hippocampus (HC) and related mesial temporal lobe (MTL) network. Resting state functional connectivity (RSFC) is considered an ideal technique in quantifying the dysfunction and maladaptation in MTL network. It is well- dem...

We have previously shown that detyrosinated microtubules (Glu MTs), which are oriented toward the direction of locomotion in motile fibroblasts, disappear from the area adjacent to cell-cell contact soon after a cell-cell collision. To identify cell surface molecules that trigger this phenomenon, we have established a system in which this and other cellular reactions to cell-cell contact can be...

This study aims to evaluate the radiosensitization effect of nedaplatin on nasopharyngeal carcinoma (NPC) cell lines with different Epstein-Barr virus (EBV) status. Human NPC cell lines CNE-2 (EBV-negative) and C666 (EBV-positive) were treated with 0-100 μg/mL nedaplatin, and inhibitory effects on cell viability and IC50 were calculated by MTS assay. We assessed changes in radiosensitivity of c...

The mechanism underlying microtubule (MT) generation in plants has been primarily studied using the cortical MT array, in which fixed-angled branching nucleation and katanin-dependent MT severing predominate. However, little is known about MT generation in the endoplasm. Here, we explored the mechanism of endoplasmic MT generation in protonemal cells of Physcomitrella patens. We developed an as...

Description Charged MTS reagents Positively charged reagent that reacts very rapidly and specifically with cysteine groups. Useful tools for protein (transporters, receptors...) structure and activity studies These reagents would fit inside a cylinder about 0.6nm in diameter and 1nm in length (Akabas . MTSEA UP996180 100mg 500mg 1g 2-Aminoethyl MethaneThioSulfonate Hydrobromide CAS [16599-33-0]...